Serodiagnostic assay of hepatitis C virus infection using viral proteins experesses in Escherichia coli

Shigehisa Mori, Showgo Ohkoshi, Makoto Hijikata, Nobuyuki Kato, Kunitada Shimotonno

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Infection with hepatitis C virus (HCV) was analyzed by an enzyme-linked immunosorbent assay based on recombinant viral proteins encoded by regions of the putative viral core, NS3, NS4 and NS5, which were expressed in E. coli. Results showed that 106 of 124 cases (85.5%) of non-A, non-B chronic hepatitis and 43 of 45 cases (95.5%) of hepatocellular carcinoma, negative for HBV marker, were positive for antibodies against at least one of these viral proteins. One of 87 healthy individuals with normal alanine aminotransferase activity was positive for antibody against only the viral core, but was negative for HCV RNA. The serum of one patient with chronic hepatitis was positive for one of these proteins, but negative for HCV RNA. These findings in combination with results on detection of HCV RNA in the sera of patients with non-A, non-B chronic hepatitis indicated that 105 of 124 cases (84.6%) were positive for HCV infection. Sera that were negative for HCV antibodies against all these proteins were also negative for HCV RNA assayed by reverse transcription followed by the polymerase chain reaction. Screening of HCV infection by detecting viral antibodies in circulating blood using all these viral proteins is useful for reducing the number of ambiguous results in screening for viral infection. Thus, this assay system may be useful diagnostic purposes.

Original languageEnglish
Pages (from-to)264-268
Number of pages5
JournalJapanese Journal of Cancer Research
Volume83
Issue number3
Publication statusPublished - Mar 1992
Externally publishedYes

Fingerprint

Viral Proteins
Virus Diseases
Hepacivirus
Escherichia coli
Chronic Hepatitis
RNA
Serum
Viral Antibodies
Hepatitis C Antibodies
Antibodies
Alanine Transaminase
Recombinant Proteins
Reverse Transcription
Hepatocellular Carcinoma
Proteins
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Infection

Keywords

  • Hepatitis C virus
  • Hepatocellular carcinoma
  • Non-A, non-B hepatitis
  • Recombinant protein
  • Serodiagnosis

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Serodiagnostic assay of hepatitis C virus infection using viral proteins experesses in Escherichia coli. / Mori, Shigehisa; Ohkoshi, Showgo; Hijikata, Makoto; Kato, Nobuyuki; Shimotonno, Kunitada.

In: Japanese Journal of Cancer Research, Vol. 83, No. 3, 03.1992, p. 264-268.

Research output: Contribution to journalArticle

Mori, Shigehisa ; Ohkoshi, Showgo ; Hijikata, Makoto ; Kato, Nobuyuki ; Shimotonno, Kunitada. / Serodiagnostic assay of hepatitis C virus infection using viral proteins experesses in Escherichia coli. In: Japanese Journal of Cancer Research. 1992 ; Vol. 83, No. 3. pp. 264-268.
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