Sequential analysis of histamine release and intracellular Ca2+ release from murine mast cells

Kenji Tasaka, Yukio Sugimoto, Mitsunobu Mio

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Stimulation of murine peritoneal mast cells with compound 48/80 at a concentration of 1 ug/ml elicited rather slow histamine release; the onset of release was observed 5 s after stimulation, and it reached a plateau at about 60 s. Both inositol-1, 4, 5-trisphosphate (IP3) and inositol-1, 4s-bisphosphate (IP2) contents increased to their maximum 5 s after stimulation. The IP3 content decreased to the control level more rapidly than that of IP2. Changes in the intracellular Ca2+ concentration of the quin 2 loaded mast cells were determined using a video-intensified microscopy system. The fluorescence intensity due to Ca-quin 2 complex increased rapidly after 48/80 stimulation in a Ca-free medium and reached the maximum at about 6-7 s. It became clear that the increase in IP3 content and the resulting Ca2+ release from the intracellular Ca store precede histamine release from murine mast cells.

Original languageEnglish
Pages (from-to)211-213
Number of pages3
JournalInternational Archives of Allergy and Immunology
Volume91
Issue number2
DOIs
Publication statusPublished - Jan 1 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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