Sequencing of a rice centromere uncovers active genes

Kiyotaka Nagaki, Zhukuan Cheng, Shu Ouyang, Paul B. Talbert, Mary Kim, Kristine M. Jones, Steven Henikoff, C. Robin Buell, Jiming Jiang

Research output: Contribution to journalArticle

385 Citations (Scopus)

Abstract

Centromeres are the last frontiers of complex eukaryotic genomes, consisting of highly repetitive sequences that resist mapping, cloning and sequencing. The centromere of rice Chromosome 8 (Cen8) has an unusually low abundance of highly repetitive satellite DNA, which allowed us to determine its sequence. A region of ∼750 kb in Cen8 binds rice CENH3, the centromere-specific H3 histone. CENH3 binding is contained within a larger region that has abundant dimethylation of histone H3 at Lys9 (H3-Lys9), consistent with Cen8 being embedded in heterochromatin. Fourteen predicted and at least four active genes are interspersed in Cen8, along with CENH3 binding sites. The retrotransposons located in and outside of the CENH3 binding domain have similar ages and structural dynamics. These results suggest that Cen8 may represent an intermediate stage in the evolution of centromeres from genic regions, as in human neocentromeres, to fully mature centromeres that accumulate megabases of homogeneous satellite arrays.

Original languageEnglish
Pages (from-to)138-145
Number of pages8
JournalNature Genetics
Volume36
Issue number2
DOIs
Publication statusPublished - Feb 2004
Externally publishedYes

Fingerprint

Centromere
Genes
Histones
Satellite DNA
Chromosomes, Human, Pair 8
Retroelements
Heterochromatin
Nucleic Acid Repetitive Sequences
Organism Cloning
Binding Sites
Oryza
Genome

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Nagaki, K., Cheng, Z., Ouyang, S., Talbert, P. B., Kim, M., Jones, K. M., ... Jiang, J. (2004). Sequencing of a rice centromere uncovers active genes. Nature Genetics, 36(2), 138-145. https://doi.org/10.1038/ng1289

Sequencing of a rice centromere uncovers active genes. / Nagaki, Kiyotaka; Cheng, Zhukuan; Ouyang, Shu; Talbert, Paul B.; Kim, Mary; Jones, Kristine M.; Henikoff, Steven; Buell, C. Robin; Jiang, Jiming.

In: Nature Genetics, Vol. 36, No. 2, 02.2004, p. 138-145.

Research output: Contribution to journalArticle

Nagaki, K, Cheng, Z, Ouyang, S, Talbert, PB, Kim, M, Jones, KM, Henikoff, S, Buell, CR & Jiang, J 2004, 'Sequencing of a rice centromere uncovers active genes', Nature Genetics, vol. 36, no. 2, pp. 138-145. https://doi.org/10.1038/ng1289
Nagaki K, Cheng Z, Ouyang S, Talbert PB, Kim M, Jones KM et al. Sequencing of a rice centromere uncovers active genes. Nature Genetics. 2004 Feb;36(2):138-145. https://doi.org/10.1038/ng1289
Nagaki, Kiyotaka ; Cheng, Zhukuan ; Ouyang, Shu ; Talbert, Paul B. ; Kim, Mary ; Jones, Kristine M. ; Henikoff, Steven ; Buell, C. Robin ; Jiang, Jiming. / Sequencing of a rice centromere uncovers active genes. In: Nature Genetics. 2004 ; Vol. 36, No. 2. pp. 138-145.
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