Sequence variations in the envelope protein of the hepatitis C virus

comparison with partial cDNA sequence of a new variant virus obtained by the polymerase chain reaction.

H. Hada, N. Koide, H. Takabatake, Tadashi Hanafusa, T. Tsuji

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3%) were significantly lower than those within each subtype (83.9-93.5%). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.

Original languageEnglish
Pages (from-to)347-355
Number of pages9
JournalActa Medica Okayama
Volume45
Issue number5
Publication statusPublished - Oct 1991

Fingerprint

Polymerase chain reaction
Protein C
Viruses
Hepacivirus
Complementary DNA
Amino Acids
Polymerase Chain Reaction
Open Reading Frames
Amino Acid Sequence
Nucleotides
Proteins
Protein Sequence Analysis
Hepatocellular Carcinoma
Genes
Genome
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sequence variations in the envelope protein of the hepatitis C virus : comparison with partial cDNA sequence of a new variant virus obtained by the polymerase chain reaction. / Hada, H.; Koide, N.; Takabatake, H.; Hanafusa, Tadashi; Tsuji, T.

In: Acta Medica Okayama, Vol. 45, No. 5, 10.1991, p. 347-355.

Research output: Contribution to journalArticle

@article{9bbd99468f354c5189a281b518d6da6e,
title = "Sequence variations in the envelope protein of the hepatitis C virus: comparison with partial cDNA sequence of a new variant virus obtained by the polymerase chain reaction.",
abstract = "It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3{\%}) were significantly lower than those within each subtype (83.9-93.5{\%}). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.",
author = "H. Hada and N. Koide and H. Takabatake and Tadashi Hanafusa and T. Tsuji",
year = "1991",
month = "10",
language = "English",
volume = "45",
pages = "347--355",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "5",

}

TY - JOUR

T1 - Sequence variations in the envelope protein of the hepatitis C virus

T2 - comparison with partial cDNA sequence of a new variant virus obtained by the polymerase chain reaction.

AU - Hada, H.

AU - Koide, N.

AU - Takabatake, H.

AU - Hanafusa, Tadashi

AU - Tsuji, T.

PY - 1991/10

Y1 - 1991/10

N2 - It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3%) were significantly lower than those within each subtype (83.9-93.5%). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.

AB - It has been reported that the envelope region located at the 3' portion of the structural protein coding region is one of the most variable regions at both nucleotide and amino acid sequence levels in the hepatitis C virus (HCV) genome. We cloned HCV cDNA fragments of an envelope protein coding region (HCVNK), which were derived from serum of a Japanese patient with hepatocellular carcinoma and were amplified by polymerase chain reaction. After determining the nucleotide sequence, deduced amino acid sequence of the envelope protein region was compared with those of six HCV strains already published (HCJ1, HCVUS, HCJ4, HCVJH, HCVJ and HCVBK). Homology analysis among the strains revealed that the seven strains were classified into two subtypes; a US subtype (HCJ1 and HCVUS) and a Japanese subtype (HCJ4, HCVJH, HCVJ, HCVBK and HCVNK), since percentage homologies between two subtypes (70.3-77.3%) were significantly lower than those within each subtype (83.9-93.5%). Detailed analysis of the amino acid sequences also indicates that the region at aa246-aa258, tentatively named intersubtype variable region-1, may distinguish the US subtype from the Japanese subtype.

UR - http://www.scopus.com/inward/record.url?scp=0026233149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026233149&partnerID=8YFLogxK

M3 - Article

VL - 45

SP - 347

EP - 355

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 5

ER -