Sequence polymorphisms in the 5′-upstream region of the fibroin H-chain gene in the silkworm, Bombyx mori

Hitoshi Ueda, Akio Hyodo, Fusaho Takei, Hiroki Sasaki, Yasumi Ohshima, Kensuke Shimura

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

DNA fragments containing the fibroin H-chain gene from two different strains Bombys mori, J-139 and Nd (2) were cloned into phage λ charon 4A. Comparison of the restriction sites in these cloned DNAs revealed that in addition to the known polymorphism in the region coding for the repetitive amino acid sequence of the fibroin H-chain [Manning and Gage, J. Biol. Chem. 255 (1980) 9451-9457], at least two other types of polymorphism were present, one around the 5′ end of the structural gene, and the other in the far upstream region of the gene. Restriction sites around the 5′ end of the gene were well conserved between these strains, but some heterogeneity, suggesting the presence of small insertions, deletions or base changes, was noted. In contrast, DNA sequences of the region 2-4 kb upstream from the 5′ end of the gene were markedly different between these two strains, indicating that either a deletion or an insertion of a DNA sequence longer than 2 kb had occurred in this region. Comparison with several other strains suggested that the observed changes in the far-upstream region were unique to the Nd(2) strain.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalGene
Volume28
Issue number2
DOIs
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Fibroins
Bombyx
Genes
Amino Acid Repetitive Sequences
Pluto
Morus
DNA
Bacteriophages

Keywords

  • bacteriophage λ
  • Charon 4A vector
  • cross-hybeidization
  • deletions
  • insertions
  • intron
  • Recombinant DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Sequence polymorphisms in the 5′-upstream region of the fibroin H-chain gene in the silkworm, Bombyx mori. / Ueda, Hitoshi; Hyodo, Akio; Takei, Fusaho; Sasaki, Hiroki; Ohshima, Yasumi; Shimura, Kensuke.

In: Gene, Vol. 28, No. 2, 1984, p. 241-248.

Research output: Contribution to journalArticle

Ueda, Hitoshi ; Hyodo, Akio ; Takei, Fusaho ; Sasaki, Hiroki ; Ohshima, Yasumi ; Shimura, Kensuke. / Sequence polymorphisms in the 5′-upstream region of the fibroin H-chain gene in the silkworm, Bombyx mori. In: Gene. 1984 ; Vol. 28, No. 2. pp. 241-248.
@article{27835a35f9ce4bf9ae4cb6c1d58e8332,
title = "Sequence polymorphisms in the 5′-upstream region of the fibroin H-chain gene in the silkworm, Bombyx mori",
abstract = "DNA fragments containing the fibroin H-chain gene from two different strains Bombys mori, J-139 and Nd (2) were cloned into phage λ charon 4A. Comparison of the restriction sites in these cloned DNAs revealed that in addition to the known polymorphism in the region coding for the repetitive amino acid sequence of the fibroin H-chain [Manning and Gage, J. Biol. Chem. 255 (1980) 9451-9457], at least two other types of polymorphism were present, one around the 5′ end of the structural gene, and the other in the far upstream region of the gene. Restriction sites around the 5′ end of the gene were well conserved between these strains, but some heterogeneity, suggesting the presence of small insertions, deletions or base changes, was noted. In contrast, DNA sequences of the region 2-4 kb upstream from the 5′ end of the gene were markedly different between these two strains, indicating that either a deletion or an insertion of a DNA sequence longer than 2 kb had occurred in this region. Comparison with several other strains suggested that the observed changes in the far-upstream region were unique to the Nd(2) strain.",
keywords = "bacteriophage λ, Charon 4A vector, cross-hybeidization, deletions, insertions, intron, Recombinant DNA",
author = "Hitoshi Ueda and Akio Hyodo and Fusaho Takei and Hiroki Sasaki and Yasumi Ohshima and Kensuke Shimura",
year = "1984",
doi = "10.1016/0378-1119(84)90262-2",
language = "English",
volume = "28",
pages = "241--248",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Sequence polymorphisms in the 5′-upstream region of the fibroin H-chain gene in the silkworm, Bombyx mori

AU - Ueda, Hitoshi

AU - Hyodo, Akio

AU - Takei, Fusaho

AU - Sasaki, Hiroki

AU - Ohshima, Yasumi

AU - Shimura, Kensuke

PY - 1984

Y1 - 1984

N2 - DNA fragments containing the fibroin H-chain gene from two different strains Bombys mori, J-139 and Nd (2) were cloned into phage λ charon 4A. Comparison of the restriction sites in these cloned DNAs revealed that in addition to the known polymorphism in the region coding for the repetitive amino acid sequence of the fibroin H-chain [Manning and Gage, J. Biol. Chem. 255 (1980) 9451-9457], at least two other types of polymorphism were present, one around the 5′ end of the structural gene, and the other in the far upstream region of the gene. Restriction sites around the 5′ end of the gene were well conserved between these strains, but some heterogeneity, suggesting the presence of small insertions, deletions or base changes, was noted. In contrast, DNA sequences of the region 2-4 kb upstream from the 5′ end of the gene were markedly different between these two strains, indicating that either a deletion or an insertion of a DNA sequence longer than 2 kb had occurred in this region. Comparison with several other strains suggested that the observed changes in the far-upstream region were unique to the Nd(2) strain.

AB - DNA fragments containing the fibroin H-chain gene from two different strains Bombys mori, J-139 and Nd (2) were cloned into phage λ charon 4A. Comparison of the restriction sites in these cloned DNAs revealed that in addition to the known polymorphism in the region coding for the repetitive amino acid sequence of the fibroin H-chain [Manning and Gage, J. Biol. Chem. 255 (1980) 9451-9457], at least two other types of polymorphism were present, one around the 5′ end of the structural gene, and the other in the far upstream region of the gene. Restriction sites around the 5′ end of the gene were well conserved between these strains, but some heterogeneity, suggesting the presence of small insertions, deletions or base changes, was noted. In contrast, DNA sequences of the region 2-4 kb upstream from the 5′ end of the gene were markedly different between these two strains, indicating that either a deletion or an insertion of a DNA sequence longer than 2 kb had occurred in this region. Comparison with several other strains suggested that the observed changes in the far-upstream region were unique to the Nd(2) strain.

KW - bacteriophage λ

KW - Charon 4A vector

KW - cross-hybeidization

KW - deletions

KW - insertions

KW - intron

KW - Recombinant DNA

UR - http://www.scopus.com/inward/record.url?scp=0021254082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021254082&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(84)90262-2

DO - 10.1016/0378-1119(84)90262-2

M3 - Article

C2 - 6329919

AN - SCOPUS:0021254082

VL - 28

SP - 241

EP - 248

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -