Sequence-independent and reversible photocontrol of transcription/ expression systems using a photosensitive nucleic acid binder

André Estévez-Torres, Cécile Crozatier, Antoine Diguet, Tomoaki Hara, Hirohide Saito, Kenichi Yoshikawa, Damien Baigl

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

To understand non-trivial biological functions, it is crucial to develop minimal synthetic models that capture their basic features. Here, we demonstrate a sequence-independent, reversible control of transcription and gene expression using a photosensitive nucleic acid binder (pNAB). By introducing a pNAB whose affinity for nucleic acids is tuned by light, in vitro RNA production, EGFP translation, and GFP expression (a set of reactions including both transcription and translation) were successfully inhibited in the dark and recovered after a short illumination at 365 nm. Our results indicate that the accessibility of the protein machinery to one or several nucleic acid binding sites can be efficiently regulated by changing the conformational/condensation state of the nucleic acid (DNA conformation or mRNA aggregation), thus regulating gene activity in an efficient, reversible, and sequence-independent manner. The possibility offered by our approach to use light to trigger various gene expression systems in a system-independent way opens interesting perspectives to study gene expression dynamics as well as to develop photocontrolled biotechnological procedures.

Original languageEnglish
Pages (from-to)12219-12223
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number30
DOIs
Publication statusPublished - Jul 28 2009
Externally publishedYes

Fingerprint

Nucleic Acids
Nucleic Acid Conformation
Gene Expression
Light
Lighting
Binding Sites
RNA
Messenger RNA
Genes
Proteins

Keywords

  • DNA conformation
  • Gene regulation
  • Light
  • RNA
  • Synthetic biology

ASJC Scopus subject areas

  • General

Cite this

Sequence-independent and reversible photocontrol of transcription/ expression systems using a photosensitive nucleic acid binder. / Estévez-Torres, André; Crozatier, Cécile; Diguet, Antoine; Hara, Tomoaki; Saito, Hirohide; Yoshikawa, Kenichi; Baigl, Damien.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 30, 28.07.2009, p. 12219-12223.

Research output: Contribution to journalArticle

Estévez-Torres, André ; Crozatier, Cécile ; Diguet, Antoine ; Hara, Tomoaki ; Saito, Hirohide ; Yoshikawa, Kenichi ; Baigl, Damien. / Sequence-independent and reversible photocontrol of transcription/ expression systems using a photosensitive nucleic acid binder. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 30. pp. 12219-12223.
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AU - Saito, Hirohide

AU - Yoshikawa, Kenichi

AU - Baigl, Damien

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