Sequence divergence and loss-of-function phenotypes of S locus F-box brothers genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia)

Hiroyuki Kakui, Masaki Kato, Koichiro Ushijima, Miyoko Kitaguchi, Shu Kato, Hidenori Sassa

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The S-RNase-based gametophytic self-incompatibility (SI) of Rosaceae, Solanaceae, and Plantaginaceae is controlled by at least two tightly linked genes located at the complex S locus; the highly polymorphic S-RNase for pistil specificity and the F-box gene (SFB/SLF) for pollen. Self-incompatibility in Prunus (Rosaceae) is considered to represent a 'self recognition by a single factor' system, because loss-of-function of SFB is associated with self-compatibility, and allelic divergence of SFB is high and comparable to that of S-RNase. In contrast, Petunia (Solanaceae) exhibits 'non-self recognition by multiple factors'. However, the distribution of 'self recognition' and 'non-self recognition' SI systems in different taxa is not clear. In addition, in 'non-self recognition' systems, a loss-of-function phenotype of pollen S is unknown. Here we analyze the divergence of SFBB genes, the multiple pollen S candidates, of a rosaceous plant Japanese pear (Pyrus pyrifolia) and show that intrahaplotypic divergence is high and comparable to the allelic diversity of S-RNase while interhaplotypic divergence is very low. Next, we analyzed loss-of-function of the SFBB1 type gene. Genetic analysis showed that pollen with the mutant haplotype S 4sm lacking SFBB1-S 4 is rejected by pistils with an otherwise compatible S 1 while it is accepted by other non-self pistils. We found that the S 5 haplotype encodes a truncated SFBB1 protein, even though S 5 pollen is accepted normally by pistils with S 1 and other non-self haplotypes. These findings suggest that Japanese pear has a 'non-self recognition by multiple factors' SI system, although it is a species of Rosaceae to which Prunus also belongs.

Original languageEnglish
Pages (from-to)1028-1038
Number of pages11
JournalPlant Journal
Volume68
Issue number6
DOIs
Publication statusPublished - Dec 2011

Fingerprint

Pyrus pyrifolia
Pyrus
Pollen
pears
pistil
Rosaceae
pollen
Phenotype
phenotype
loci
Haplotypes
Solanaceae
haplotypes
Genes
Prunus
genes
Petunia
Plantaginaceae
Protein S
genetic techniques and protocols

Keywords

  • F-box protein
  • Japanese pear
  • pollen
  • Rosaceae
  • S-RNase
  • self-incompatibility

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Sequence divergence and loss-of-function phenotypes of S locus F-box brothers genes are consistent with non-self recognition by multiple pollen determinants in self-incompatibility of Japanese pear (Pyrus pyrifolia). / Kakui, Hiroyuki; Kato, Masaki; Ushijima, Koichiro; Kitaguchi, Miyoko; Kato, Shu; Sassa, Hidenori.

In: Plant Journal, Vol. 68, No. 6, 12.2011, p. 1028-1038.

Research output: Contribution to journalArticle

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abstract = "The S-RNase-based gametophytic self-incompatibility (SI) of Rosaceae, Solanaceae, and Plantaginaceae is controlled by at least two tightly linked genes located at the complex S locus; the highly polymorphic S-RNase for pistil specificity and the F-box gene (SFB/SLF) for pollen. Self-incompatibility in Prunus (Rosaceae) is considered to represent a 'self recognition by a single factor' system, because loss-of-function of SFB is associated with self-compatibility, and allelic divergence of SFB is high and comparable to that of S-RNase. In contrast, Petunia (Solanaceae) exhibits 'non-self recognition by multiple factors'. However, the distribution of 'self recognition' and 'non-self recognition' SI systems in different taxa is not clear. In addition, in 'non-self recognition' systems, a loss-of-function phenotype of pollen S is unknown. Here we analyze the divergence of SFBB genes, the multiple pollen S candidates, of a rosaceous plant Japanese pear (Pyrus pyrifolia) and show that intrahaplotypic divergence is high and comparable to the allelic diversity of S-RNase while interhaplotypic divergence is very low. Next, we analyzed loss-of-function of the SFBB1 type gene. Genetic analysis showed that pollen with the mutant haplotype S 4sm lacking SFBB1-S 4 is rejected by pistils with an otherwise compatible S 1 while it is accepted by other non-self pistils. We found that the S 5 haplotype encodes a truncated SFBB1 protein, even though S 5 pollen is accepted normally by pistils with S 1 and other non-self haplotypes. These findings suggest that Japanese pear has a 'non-self recognition by multiple factors' SI system, although it is a species of Rosaceae to which Prunus also belongs.",
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