Abstract
The nucleotide sequence of the Xestia c-nigrum granulovirus (XcGV) genome was determined and found to comprise 178,733 bases with a G+C content of 40.7%. It contained 181 putative genes of 150 nucleotides or greater that showed minimal overlap. Eighty-four of these putative genes, which collectively accounted for 43% of the genome, are homologs of genes previously identified in the Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) genome. These homologs showed on average 33% amine acid sequence identity to those from AcMNPV. Several genes reported to have major roles in AcMNPV biology including ie-2, gp64, and egt were not found in the XcGV genome. However, open reading frames with homology to DNA ligase, two DNA helicases (one similar to a yeast mitochondrial helicase and the other to a putative AcMNPV helicase), and four enhancins (virus enhancing factors) were found. In addition, several ORFs are repeated; there are 7 genes related to AcMNPV orf2, 4 genes related to AcMNPV orf145/150, and a number of repeated genes unique to XcGV. Eight major repeated sequences (XcGV hrs) that are similar to sequences found in the Trichoplusia ni GV genome (TnGV) were found.
| Original language | English |
|---|---|
| Pages (from-to) | 277-297 |
| Number of pages | 21 |
| Journal | Virology |
| Volume | 262 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 30 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Sequence analysis of the Xestia c-nigrum granulovirus genome. / Hayakawa, Tohru; Ko, Rinkei; Okano, Kazuhiro; Seong, Su Il; Goto, Chie; Maeda, Susumu.
In: Virology, Vol. 262, No. 2, 30.09.1999, p. 277-297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sequence analysis of the Xestia c-nigrum granulovirus genome
AU - Hayakawa, Tohru
AU - Ko, Rinkei
AU - Okano, Kazuhiro
AU - Seong, Su Il
AU - Goto, Chie
AU - Maeda, Susumu
PY - 1999/9/30
Y1 - 1999/9/30
N2 - The nucleotide sequence of the Xestia c-nigrum granulovirus (XcGV) genome was determined and found to comprise 178,733 bases with a G+C content of 40.7%. It contained 181 putative genes of 150 nucleotides or greater that showed minimal overlap. Eighty-four of these putative genes, which collectively accounted for 43% of the genome, are homologs of genes previously identified in the Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) genome. These homologs showed on average 33% amine acid sequence identity to those from AcMNPV. Several genes reported to have major roles in AcMNPV biology including ie-2, gp64, and egt were not found in the XcGV genome. However, open reading frames with homology to DNA ligase, two DNA helicases (one similar to a yeast mitochondrial helicase and the other to a putative AcMNPV helicase), and four enhancins (virus enhancing factors) were found. In addition, several ORFs are repeated; there are 7 genes related to AcMNPV orf2, 4 genes related to AcMNPV orf145/150, and a number of repeated genes unique to XcGV. Eight major repeated sequences (XcGV hrs) that are similar to sequences found in the Trichoplusia ni GV genome (TnGV) were found.
AB - The nucleotide sequence of the Xestia c-nigrum granulovirus (XcGV) genome was determined and found to comprise 178,733 bases with a G+C content of 40.7%. It contained 181 putative genes of 150 nucleotides or greater that showed minimal overlap. Eighty-four of these putative genes, which collectively accounted for 43% of the genome, are homologs of genes previously identified in the Autographa californica multinucleocapsid nucleopolyhedrovirus (AcMNPV) genome. These homologs showed on average 33% amine acid sequence identity to those from AcMNPV. Several genes reported to have major roles in AcMNPV biology including ie-2, gp64, and egt were not found in the XcGV genome. However, open reading frames with homology to DNA ligase, two DNA helicases (one similar to a yeast mitochondrial helicase and the other to a putative AcMNPV helicase), and four enhancins (virus enhancing factors) were found. In addition, several ORFs are repeated; there are 7 genes related to AcMNPV orf2, 4 genes related to AcMNPV orf145/150, and a number of repeated genes unique to XcGV. Eight major repeated sequences (XcGV hrs) that are similar to sequences found in the Trichoplusia ni GV genome (TnGV) were found.
UR - http://www.scopus.com/inward/record.url?scp=0033619202&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033619202&partnerID=8YFLogxK
U2 - 10.1006/viro.1999.9894
DO - 10.1006/viro.1999.9894
M3 - Article
C2 - 10502508
AN - SCOPUS:0033619202
VL - 262
SP - 277
EP - 297
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -