Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa

Nobuhiro Suzuki, Yuzuko Watanabe, Tomonobu Kusano, Yoshichika Kitagawa

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa.

Original languageEnglish
Pages (from-to)455-459
Number of pages5
JournalVirology
Volume179
Issue number1
DOIs
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Reoviridae
Viral Core Proteins
Sequence Analysis
Peptide Fragments
Conserved Sequence
Open Reading Frames
Staphylococcus aureus
Amino Acid Sequence
Nucleotides
Genome
Amino Acids
Peptides
Oryza
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa. / Suzuki, Nobuhiro; Watanabe, Yuzuko; Kusano, Tomonobu; Kitagawa, Yoshichika.

In: Virology, Vol. 179, No. 1, 1990, p. 455-459.

Research output: Contribution to journalArticle

Suzuki, Nobuhiro ; Watanabe, Yuzuko ; Kusano, Tomonobu ; Kitagawa, Yoshichika. / Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa. In: Virology. 1990 ; Vol. 179, No. 1. pp. 455-459.
@article{020c662c43eb403daab0a9bf54e3bb04,
title = "Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa",
abstract = "The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa.",
author = "Nobuhiro Suzuki and Yuzuko Watanabe and Tomonobu Kusano and Yoshichika Kitagawa",
year = "1990",
doi = "10.1016/0042-6822(90)90314-H",
language = "English",
volume = "179",
pages = "455--459",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Sequence analysis of the rice dwarf phytoreovirus segment s3 transcript encoding for the major structural core protein of 114 kDa

AU - Suzuki, Nobuhiro

AU - Watanabe, Yuzuko

AU - Kusano, Tomonobu

AU - Kitagawa, Yoshichika

PY - 1990

Y1 - 1990

N2 - The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa.

AB - The primary structure of rice dwarf phytoreovirus (RDV) genome segment S3 was determined. RDV S3 consists of 3195 nucleotides. A 14-bp segment-specific inverted repeat is located immediately adjacent to the conserved terminal sequence (5′GGCAAA---UGAU3′). A single long open reading frame encoding for 1019 amino acids with an Mr of 114,289 is also identified. In order to investigate the localization of the predicted polypeptide, we determined the amino acid sequence of the 26-kDa peptide fragment obtained from the structural core protein digested by Staphylococcus aureus V8 protease. The sequence of the fragment was found in the translational product presumed from the nucleotide sequence of RDV S3, indicating that RDV S3 encodes the major structural core protein of 114 kDa.

UR - http://www.scopus.com/inward/record.url?scp=0025036274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025036274&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(90)90314-H

DO - 10.1016/0042-6822(90)90314-H

M3 - Article

VL - 179

SP - 455

EP - 459

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -