Sequence analysis of the flgA gene and its adjacent region in Salmonella typhimurium, and identification of another flagellar gene, flgN

Kutsukake Kazuhiro, Okada Tsutomu, Yokoseki Tatsuki, Iino Tetsuo

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The flagellar genes flgA and flgM are located at the terminus of the region-I flagellar gene cluster on the chromosome of Salmonella typhimurium. The flgA gene is involved in P-ring formation of the flagellar basal body, whereas flgM encodes the anti-sigma factor which acts as a negative regulator of the flagellar regulon. The nucleotide sequence of the DNA fragment containing these flagellar genes and the adjacent region was determined. The flgA gene was found to encode a 219-amino-acid (aa) protein of 23 556 Da. The N-terminal region of FlgA has the characteristics of a typical signal sequence, suggesting that FlgA may function in the periplasmic space where P-ring assembly takes place. The flgM gene was found to constitute an operon together with an ORF which encodes a 140-aa protein of 15899 Da. A gene disruption mutant was constructed by inserting a cat gene cartridge into the ORF on the chromosome. This mutant showed only weak motility, indicating that the product of the ORF is involved in flagellar formation. Therefore, this ORF was designated as flgN. Electron microscopic observation revealed that most of the flagellar structures produced by the flgN mutant are hook-basal body complexes lacking the filament portions. Based on these results, we concluded that the flgN product is required for the efficient initiation of filament assembly.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalGene
Volume143
Issue number1
DOIs
Publication statusPublished - May 27 1994
Externally publishedYes

Fingerprint

Salmonella typhimurium
Sequence Analysis
Open Reading Frames
Genes
Basal Bodies
Chromosomes
Sigma Factor
Amino Acids
Regulon
Periplasm
Operon
Multigene Family
Protein Sorting Signals
Proteins
Cats
Electrons
DNA

Keywords

  • anti-sigma factor
  • DNA sequencing
  • filament assembly
  • gene disruption
  • P-ring formation

ASJC Scopus subject areas

  • Genetics

Cite this

Sequence analysis of the flgA gene and its adjacent region in Salmonella typhimurium, and identification of another flagellar gene, flgN. / Kazuhiro, Kutsukake; Tsutomu, Okada; Tatsuki, Yokoseki; Tetsuo, Iino.

In: Gene, Vol. 143, No. 1, 27.05.1994, p. 49-54.

Research output: Contribution to journalArticle

Kazuhiro, Kutsukake ; Tsutomu, Okada ; Tatsuki, Yokoseki ; Tetsuo, Iino. / Sequence analysis of the flgA gene and its adjacent region in Salmonella typhimurium, and identification of another flagellar gene, flgN. In: Gene. 1994 ; Vol. 143, No. 1. pp. 49-54.
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