Sequence analysis of operator mutants of the phase-1 flagellin-encoding gene, flic, in salmonella typhimurium

Yoshihiro H. Inoue, Kazuhiro Kutsukake, Tetsuo Iino, Shigeru Yamaguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In phase-2 cells of diphasic Salmonella strains, expression of the phase-1 flagellin-encoding gene, fliC, is repressed by the repressor encoded by the fljA gene. Nine operator-constitutive (Oc) mutants of fliC were isolated from S. typhimurium by selecting those which could express fliC in the presence of the repressor. Among them, eight mutants could express fliC both m the presence and the absence of the repressor, whereas the ninth one could express only in the presence of the repressor. Nucleotide sequence analysis revealed that the Oc mutations of the former type were all located between bp 7 and 20 upstream from the coding region of fliC, which suggests that this region may correspond to the operator for fliC. The latter mutant was found to have a tandem duplication of 28 bp which contains a part of the operator sequence, and seems to require the represser to activate fliC expression.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalGene
Volume85
Issue number1
DOIs
Publication statusPublished - Dec 21 1989
Externally publishedYes

Fingerprint

Flagellin
Salmonella typhimurium
Sequence Analysis
Salmonella
Genes
Mutation

Keywords

  • cloning
  • constitutive mutant
  • inverted repeat
  • nucleotide sequence
  • Recombinant DNA
  • repression
  • tandem duplication
  • transcription

ASJC Scopus subject areas

  • Genetics

Cite this

Sequence analysis of operator mutants of the phase-1 flagellin-encoding gene, flic, in salmonella typhimurium. / Inoue, Yoshihiro H.; Kutsukake, Kazuhiro; Iino, Tetsuo; Yamaguchi, Shigeru.

In: Gene, Vol. 85, No. 1, 21.12.1989, p. 221-226.

Research output: Contribution to journalArticle

Inoue, Yoshihiro H. ; Kutsukake, Kazuhiro ; Iino, Tetsuo ; Yamaguchi, Shigeru. / Sequence analysis of operator mutants of the phase-1 flagellin-encoding gene, flic, in salmonella typhimurium. In: Gene. 1989 ; Vol. 85, No. 1. pp. 221-226.
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AU - Iino, Tetsuo

AU - Yamaguchi, Shigeru

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N2 - In phase-2 cells of diphasic Salmonella strains, expression of the phase-1 flagellin-encoding gene, fliC, is repressed by the repressor encoded by the fljA gene. Nine operator-constitutive (Oc) mutants of fliC were isolated from S. typhimurium by selecting those which could express fliC in the presence of the repressor. Among them, eight mutants could express fliC both m the presence and the absence of the repressor, whereas the ninth one could express only in the presence of the repressor. Nucleotide sequence analysis revealed that the Oc mutations of the former type were all located between bp 7 and 20 upstream from the coding region of fliC, which suggests that this region may correspond to the operator for fliC. The latter mutant was found to have a tandem duplication of 28 bp which contains a part of the operator sequence, and seems to require the represser to activate fliC expression.

AB - In phase-2 cells of diphasic Salmonella strains, expression of the phase-1 flagellin-encoding gene, fliC, is repressed by the repressor encoded by the fljA gene. Nine operator-constitutive (Oc) mutants of fliC were isolated from S. typhimurium by selecting those which could express fliC in the presence of the repressor. Among them, eight mutants could express fliC both m the presence and the absence of the repressor, whereas the ninth one could express only in the presence of the repressor. Nucleotide sequence analysis revealed that the Oc mutations of the former type were all located between bp 7 and 20 upstream from the coding region of fliC, which suggests that this region may correspond to the operator for fliC. The latter mutant was found to have a tandem duplication of 28 bp which contains a part of the operator sequence, and seems to require the represser to activate fliC expression.

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