Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey

Nobuya Ohara, Kazuhiko Hayashi, Norihiro Teramoto, Takashi Oka, Koji Fujimoto, Yasuhiro Yoshikawa, Esmeralda Castanos-Velez, Peter Biberfeld, Tadaatsu Akagi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Objectives: The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is an important protein for immortalization and tumorigenesis of infected cells. EBNA-1 gene variants may play a role in tumorigenesis. We determined the nucleotide and amino acid (aa) sequences of EBNA-1 in EBV-related herpesviruses from cynomolgus monkeys (cynomolgus-EBV) which induced malignant lymphomas in its natural host and in rabbits, and compared them with sequences of EBV and other lymphocryptoviruses (LCVs). Methods: Polymerase chain reaction and direct sequencing methods were performed using extracted DNA from cynomolgus-EBV-infected cell lines. Results: The amino acid sequences of cynomolgus-EBV EBNA-1 from two cell lines (Si-IIA: 588 aa; Ts-B6: 619 aa) which are antigenically cross-reactive to human EBV EBNA-1 showed homology with human EBV (Si-IIA: 53%; Ts-B6: 58%) and other LCVs from baboons (54 and 52%) and rhesus monkeys (60 and 58%), especially in the C- terminal unique domain. Homology of the EBNA-1 sequence between Si-IIA and Ts-B6 was 92%. The sequence difference between EBV and the related LCVs was manifested mainly in the length of the internal repeat 3-corresponding region, which contains serine in the glycine/alanine repeat region of nonhuman LCVs. Conclusion: Sequence variation of cynomolgus-EBV EBNA-1 from different cell lines was observed. However, their sequences show a relatively high homology with human EBV and share the common features of EBNA-1 of EBV and other LCVs. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)102-106
Number of pages5
Issue number2
Publication statusPublished - Aug 2000


  • Cynomolgus-EBV
  • EBNA-1
  • Epstein-Barr virus
  • Lymphocryptovirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases


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