Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey

Nobuya Ohara, Kazuhiko Hayashi, Norihiro Teramoto, Takashi Oka, Koji Fujimoto, Yasuhiro Yoshikawa, Esmeralda Castanos-Velez, Peter Biberfeld, Tadaatsu Akagi

Research output: Contribution to journalArticle

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Abstract

Objectives: The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is an important protein for immortalization and tumorigenesis of infected cells. EBNA-1 gene variants may play a role in tumorigenesis. We determined the nucleotide and amino acid (aa) sequences of EBNA-1 in EBV-related herpesviruses from cynomolgus monkeys (cynomolgus-EBV) which induced malignant lymphomas in its natural host and in rabbits, and compared them with sequences of EBV and other lymphocryptoviruses (LCVs). Methods: Polymerase chain reaction and direct sequencing methods were performed using extracted DNA from cynomolgus-EBV-infected cell lines. Results: The amino acid sequences of cynomolgus-EBV EBNA-1 from two cell lines (Si-IIA: 588 aa; Ts-B6: 619 aa) which are antigenically cross-reactive to human EBV EBNA-1 showed homology with human EBV (Si-IIA: 53%; Ts-B6: 58%) and other LCVs from baboons (54 and 52%) and rhesus monkeys (60 and 58%), especially in the C- terminal unique domain. Homology of the EBNA-1 sequence between Si-IIA and Ts-B6 was 92%. The sequence difference between EBV and the related LCVs was manifested mainly in the length of the internal repeat 3-corresponding region, which contains serine in the glycine/alanine repeat region of nonhuman LCVs. Conclusion: Sequence variation of cynomolgus-EBV EBNA-1 from different cell lines was observed. However, their sequences show a relatively high homology with human EBV and share the common features of EBNA-1 of EBV and other LCVs. Copyright (C) 2000 S. Karger AG, Basel.

Original languageEnglish
Pages (from-to)102-106
Number of pages5
JournalIntervirology
Volume43
Issue number2
Publication statusPublished - Aug 2000

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Epstein-Barr Virus Nuclear Antigens
Macaca fascicularis
Herpesviridae
Human Herpesvirus 4
Sequence Analysis
Lymphocryptovirus
Cell Line
Amino Acid Sequence
Carcinogenesis
Amino Acids
Papio
Macaca mulatta
Alanine
Glycine
Serine
Lymphoma
Nucleotides

Keywords

  • Cynomolgus-EBV
  • EBNA-1
  • Epstein-Barr virus
  • Lymphocryptovirus

ASJC Scopus subject areas

  • Virology

Cite this

Ohara, N., Hayashi, K., Teramoto, N., Oka, T., Fujimoto, K., Yoshikawa, Y., ... Akagi, T. (2000). Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey. Intervirology, 43(2), 102-106.

Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey. / Ohara, Nobuya; Hayashi, Kazuhiko; Teramoto, Norihiro; Oka, Takashi; Fujimoto, Koji; Yoshikawa, Yasuhiro; Castanos-Velez, Esmeralda; Biberfeld, Peter; Akagi, Tadaatsu.

In: Intervirology, Vol. 43, No. 2, 08.2000, p. 102-106.

Research output: Contribution to journalArticle

Ohara, N, Hayashi, K, Teramoto, N, Oka, T, Fujimoto, K, Yoshikawa, Y, Castanos-Velez, E, Biberfeld, P & Akagi, T 2000, 'Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey', Intervirology, vol. 43, no. 2, pp. 102-106.
Ohara N, Hayashi K, Teramoto N, Oka T, Fujimoto K, Yoshikawa Y et al. Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey. Intervirology. 2000 Aug;43(2):102-106.
Ohara, Nobuya ; Hayashi, Kazuhiko ; Teramoto, Norihiro ; Oka, Takashi ; Fujimoto, Koji ; Yoshikawa, Yasuhiro ; Castanos-Velez, Esmeralda ; Biberfeld, Peter ; Akagi, Tadaatsu. / Sequence analysis and variation of EBNA-1 in Epstein-Barr virus-related herpesvirus of cynomolgus monkey. In: Intervirology. 2000 ; Vol. 43, No. 2. pp. 102-106.
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abstract = "Objectives: The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is an important protein for immortalization and tumorigenesis of infected cells. EBNA-1 gene variants may play a role in tumorigenesis. We determined the nucleotide and amino acid (aa) sequences of EBNA-1 in EBV-related herpesviruses from cynomolgus monkeys (cynomolgus-EBV) which induced malignant lymphomas in its natural host and in rabbits, and compared them with sequences of EBV and other lymphocryptoviruses (LCVs). Methods: Polymerase chain reaction and direct sequencing methods were performed using extracted DNA from cynomolgus-EBV-infected cell lines. Results: The amino acid sequences of cynomolgus-EBV EBNA-1 from two cell lines (Si-IIA: 588 aa; Ts-B6: 619 aa) which are antigenically cross-reactive to human EBV EBNA-1 showed homology with human EBV (Si-IIA: 53{\%}; Ts-B6: 58{\%}) and other LCVs from baboons (54 and 52{\%}) and rhesus monkeys (60 and 58{\%}), especially in the C- terminal unique domain. Homology of the EBNA-1 sequence between Si-IIA and Ts-B6 was 92{\%}. The sequence difference between EBV and the related LCVs was manifested mainly in the length of the internal repeat 3-corresponding region, which contains serine in the glycine/alanine repeat region of nonhuman LCVs. Conclusion: Sequence variation of cynomolgus-EBV EBNA-1 from different cell lines was observed. However, their sequences show a relatively high homology with human EBV and share the common features of EBNA-1 of EBV and other LCVs. Copyright (C) 2000 S. Karger AG, Basel.",
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AU - Ohara, Nobuya

AU - Hayashi, Kazuhiko

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AU - Oka, Takashi

AU - Fujimoto, Koji

AU - Yoshikawa, Yasuhiro

AU - Castanos-Velez, Esmeralda

AU - Biberfeld, Peter

AU - Akagi, Tadaatsu

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N2 - Objectives: The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is an important protein for immortalization and tumorigenesis of infected cells. EBNA-1 gene variants may play a role in tumorigenesis. We determined the nucleotide and amino acid (aa) sequences of EBNA-1 in EBV-related herpesviruses from cynomolgus monkeys (cynomolgus-EBV) which induced malignant lymphomas in its natural host and in rabbits, and compared them with sequences of EBV and other lymphocryptoviruses (LCVs). Methods: Polymerase chain reaction and direct sequencing methods were performed using extracted DNA from cynomolgus-EBV-infected cell lines. Results: The amino acid sequences of cynomolgus-EBV EBNA-1 from two cell lines (Si-IIA: 588 aa; Ts-B6: 619 aa) which are antigenically cross-reactive to human EBV EBNA-1 showed homology with human EBV (Si-IIA: 53%; Ts-B6: 58%) and other LCVs from baboons (54 and 52%) and rhesus monkeys (60 and 58%), especially in the C- terminal unique domain. Homology of the EBNA-1 sequence between Si-IIA and Ts-B6 was 92%. The sequence difference between EBV and the related LCVs was manifested mainly in the length of the internal repeat 3-corresponding region, which contains serine in the glycine/alanine repeat region of nonhuman LCVs. Conclusion: Sequence variation of cynomolgus-EBV EBNA-1 from different cell lines was observed. However, their sequences show a relatively high homology with human EBV and share the common features of EBNA-1 of EBV and other LCVs. Copyright (C) 2000 S. Karger AG, Basel.

AB - Objectives: The Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA-1) is an important protein for immortalization and tumorigenesis of infected cells. EBNA-1 gene variants may play a role in tumorigenesis. We determined the nucleotide and amino acid (aa) sequences of EBNA-1 in EBV-related herpesviruses from cynomolgus monkeys (cynomolgus-EBV) which induced malignant lymphomas in its natural host and in rabbits, and compared them with sequences of EBV and other lymphocryptoviruses (LCVs). Methods: Polymerase chain reaction and direct sequencing methods were performed using extracted DNA from cynomolgus-EBV-infected cell lines. Results: The amino acid sequences of cynomolgus-EBV EBNA-1 from two cell lines (Si-IIA: 588 aa; Ts-B6: 619 aa) which are antigenically cross-reactive to human EBV EBNA-1 showed homology with human EBV (Si-IIA: 53%; Ts-B6: 58%) and other LCVs from baboons (54 and 52%) and rhesus monkeys (60 and 58%), especially in the C- terminal unique domain. Homology of the EBNA-1 sequence between Si-IIA and Ts-B6 was 92%. The sequence difference between EBV and the related LCVs was manifested mainly in the length of the internal repeat 3-corresponding region, which contains serine in the glycine/alanine repeat region of nonhuman LCVs. Conclusion: Sequence variation of cynomolgus-EBV EBNA-1 from different cell lines was observed. However, their sequences show a relatively high homology with human EBV and share the common features of EBNA-1 of EBV and other LCVs. Copyright (C) 2000 S. Karger AG, Basel.

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