TY - JOUR
T1 - Separation of Peptide Groups with Definite Characteristics from Enzymatic Protein Hydrolysate
AU - Adachi, Shuji
AU - Kimura, Yukitaka
AU - Murakami, Koji
AU - Matsuno, Ryuichi
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1991
Y1 - 1991
N2 - For producing peptides with definite characteristics from a casein hydrolysate treated consecutively with thermolysin and papain, the hydrolysate was separated through two chromatographic steps into four peptide groups with high or low contents of aromatic amino acids (AAA) and branched chain amino acids (BCAA). The hydrolysate was first separated into AAA-poor and -rich peptide groups (one-half and 3 to 4 times as high as the AAA content of the hydrolysate) by both a conventional batch chromatography with Sephadex G-15 and a continuous one, with the same resin, of a simulated moving-bed type. The AAA-poor peptide group was further separated into a BCAA-rich (about 1.5-fold) peptide group and another one with an increased content of hydrophilic amino acids by batch chromatography with Toyopearl HW-40S.
AB - For producing peptides with definite characteristics from a casein hydrolysate treated consecutively with thermolysin and papain, the hydrolysate was separated through two chromatographic steps into four peptide groups with high or low contents of aromatic amino acids (AAA) and branched chain amino acids (BCAA). The hydrolysate was first separated into AAA-poor and -rich peptide groups (one-half and 3 to 4 times as high as the AAA content of the hydrolysate) by both a conventional batch chromatography with Sephadex G-15 and a continuous one, with the same resin, of a simulated moving-bed type. The AAA-poor peptide group was further separated into a BCAA-rich (about 1.5-fold) peptide group and another one with an increased content of hydrophilic amino acids by batch chromatography with Toyopearl HW-40S.
UR - http://www.scopus.com/inward/record.url?scp=85007781081&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007781081&partnerID=8YFLogxK
U2 - 10.1271/bbb1961.55.933
DO - 10.1271/bbb1961.55.933
M3 - Article
AN - SCOPUS:85007781081
VL - 55
SP - 933
EP - 940
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 4
ER -