Separation of peptide groups with definite characteristics from enzymatic protein hydrolysate

For producing peptides with definite characteristics from a casein hydrolysate treated consecutively with thermolysin and papain, the hydrolysate was separated through two chromatographic steps into four peptide groups with high or low contents of aromatic amino acids (AAA) and branched chain amino acids (BCAA). The hydrolysate was first separated into AAA-poor and -rich peptide groups (one-half and 3 to 4 times as high as the AAA content of the hydrolysate) by both a conventional batch chromatography with Sephadex G-15 and a continuous one, with the same resin, of a simulated moving-bed type. The AAA-poor peptide group was further separated into a BCAA-rich (about 1.5-fold) peptide group and another one with an increased content of hydrophilic amino acids by batch chromatography with Toyopearl HW-40S.