Separate evaluation of intestinal and hepatic metabolism of three benzodiazepines in rats with cannulated portal and jugular veins: Comparison with the profile in non-cannulated mice

J. Kuze, T. Mutoh, T. Takenaka, K. Morisaki, H. Nakura, N. Hanioka, S. Narimatsu

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Pharmacokinetic analyses of three kinds of benzodiazepinesmidazolam (MDZ), triazolam (TRZ) and alprezolam (APZ)were performed in rats with cannulated portal and jugular veins. Each drug was administered to the double-cannulated rats, and pharmacokinetic data for the parent drugs and their 1′- and 4-hydroxylated metabolites were compared with those obtained in non-cannulated mice. In bioavailability, the drugs ranked APZ >> TRZ MDZ in rats, and APZ > TRZ >> MDZ in mice, with the values for MDZ remarkably different between rats and mice (19% in rats versus 2.3% in mice). In contrast, hepatic availability (Fh) was similar (APZ > TRZ > MDZ) in both species. Highly significant relationships were found between the ratio of the area under the plasma concentrationtime curve (AUC) for the parent drugs in portal blood (AUCpor) to that in systemic blood (AUCsys) and Fh in rats and mice. The double-cannulated rat is useful for estimating the hepatic availability of drug candidates by determining the AUC values for the parent drugs in portal and systemic blood samples.

Original languageEnglish
Pages (from-to)871-880
Number of pages10
JournalXenobiotica
Volume39
Issue number11
DOIs
Publication statusPublished - Nov 1 2009

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Keywords

  • Alprezolam
  • Availability
  • Double-cannulation
  • Midazolam
  • Mouse
  • Portal and systemic blood
  • Rat
  • Triazolam

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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