Sensitivity of anticancer drugs in NIH3T3 cells transfected with oncogenes accompanied by pSV2neo vector

X. S. Gao, Jun-Ichi Asaumi, S. Kawasaki, K. Nishikawa, Masahiro Kuroda, Yoshihiro Takeda, Y. Hiraki, M. Ihara, T. Ohnishi

Research output: Contribution to journalArticle

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Abstract

NIH3T3 cells and NIH3T3 cell lines, which were transfected with several oncogenes accompanied by pSV2neo vector, were observed for their survival rate when treated with commonly used anticancer drugs. The survival rates in the cell line transfected with the pSV2neo vector only did not differ significantly from that of parental NIH3T3 cells against bleomycin, nimustine and adriamycin, but it was significantly more resistant to cisplatin and more sensitive to mitomycin C. Therefore, the survival rate in each transfectant was compared with that in the pSV2neo transfectant. The Val-12, v-Ha- ras, v-int-2, v-erbB or v-abl transfectants accompanied by pSV2neo vector were significantly more sensitive to cisplatin than transfection with pSV2neo vector only. The Val-12, v-Ha-ras, v-int-2 and v-abl transfectants were significantly more sensitive, and the v-erbB transfectant more resistant to bleomycin than the pSV2neo transfectant. The v-int-2, v-erbB, frg, v-raf and v-myc transfectants were more sensitive, and the v-Ha-ras transfectant more resistant to nimustine than the pSV2neo transfectant. The N-ras, v-Ha-ras, v-sis, v-int, v-abl and v-myc transfectants were significantly more sensitive to adriamycin than the pSV2neo transfectant. The v-sis and v-int-2 transfectant were more sensitive, and the c-Ki-ras, Val-12, v-erbB and the v-src transfectant more resistant to mitomycin C than the pSV2neo transfectant. Thus there was no relationship between the drug sensitivity and the location of oncogenes, but the transfection was association with either increased or decreased sensitivity to a number of commonly used anticancer drugs. Therefore it may be important to take into consideration or overexpression of oncogenes in cancer chemotherapy.

Original languageEnglish
Pages (from-to)1911-1914
Number of pages4
JournalAnticancer Research
Volume15
Issue number5 B
Publication statusPublished - 1995

Fingerprint

Nimustine
Oncogenes
Bleomycin
Mitomycin
Doxorubicin
Cisplatin
Transfection
Pharmaceutical Preparations
Cell Line
Drug Therapy
Neoplasms

Keywords

  • Anticancer drug sensitivity
  • NIH3T3 cells
  • Oncogene
  • pSV2neo vector
  • Transfectant

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sensitivity of anticancer drugs in NIH3T3 cells transfected with oncogenes accompanied by pSV2neo vector. / Gao, X. S.; Asaumi, Jun-Ichi; Kawasaki, S.; Nishikawa, K.; Kuroda, Masahiro; Takeda, Yoshihiro; Hiraki, Y.; Ihara, M.; Ohnishi, T.

In: Anticancer Research, Vol. 15, No. 5 B, 1995, p. 1911-1914.

Research output: Contribution to journalArticle

Gao, XS, Asaumi, J-I, Kawasaki, S, Nishikawa, K, Kuroda, M, Takeda, Y, Hiraki, Y, Ihara, M & Ohnishi, T 1995, 'Sensitivity of anticancer drugs in NIH3T3 cells transfected with oncogenes accompanied by pSV2neo vector', Anticancer Research, vol. 15, no. 5 B, pp. 1911-1914.
Gao, X. S. ; Asaumi, Jun-Ichi ; Kawasaki, S. ; Nishikawa, K. ; Kuroda, Masahiro ; Takeda, Yoshihiro ; Hiraki, Y. ; Ihara, M. ; Ohnishi, T. / Sensitivity of anticancer drugs in NIH3T3 cells transfected with oncogenes accompanied by pSV2neo vector. In: Anticancer Research. 1995 ; Vol. 15, No. 5 B. pp. 1911-1914.
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abstract = "NIH3T3 cells and NIH3T3 cell lines, which were transfected with several oncogenes accompanied by pSV2neo vector, were observed for their survival rate when treated with commonly used anticancer drugs. The survival rates in the cell line transfected with the pSV2neo vector only did not differ significantly from that of parental NIH3T3 cells against bleomycin, nimustine and adriamycin, but it was significantly more resistant to cisplatin and more sensitive to mitomycin C. Therefore, the survival rate in each transfectant was compared with that in the pSV2neo transfectant. The Val-12, v-Ha- ras, v-int-2, v-erbB or v-abl transfectants accompanied by pSV2neo vector were significantly more sensitive to cisplatin than transfection with pSV2neo vector only. The Val-12, v-Ha-ras, v-int-2 and v-abl transfectants were significantly more sensitive, and the v-erbB transfectant more resistant to bleomycin than the pSV2neo transfectant. The v-int-2, v-erbB, frg, v-raf and v-myc transfectants were more sensitive, and the v-Ha-ras transfectant more resistant to nimustine than the pSV2neo transfectant. The N-ras, v-Ha-ras, v-sis, v-int, v-abl and v-myc transfectants were significantly more sensitive to adriamycin than the pSV2neo transfectant. The v-sis and v-int-2 transfectant were more sensitive, and the c-Ki-ras, Val-12, v-erbB and the v-src transfectant more resistant to mitomycin C than the pSV2neo transfectant. Thus there was no relationship between the drug sensitivity and the location of oncogenes, but the transfection was association with either increased or decreased sensitivity to a number of commonly used anticancer drugs. Therefore it may be important to take into consideration or overexpression of oncogenes in cancer chemotherapy.",
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AU - Asaumi, Jun-Ichi

AU - Kawasaki, S.

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AU - Kuroda, Masahiro

AU - Takeda, Yoshihiro

AU - Hiraki, Y.

AU - Ihara, M.

AU - Ohnishi, T.

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