Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant

Minoru Noda, Prinya Lorchirachoonkul, Toshinori Shimanouchi, Kaoru Yamashita, Hiroshi Umakoshi, Ryouichi Kuboi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect the existence of proteins. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully resulted in a prominent improvement in sensitivity by 129-folds of magnitude. This is because the modulation of conformation of the target proteins, carbonic anhydrase from bovine (CAB) and lysozyme could contribute to the promotion of the interaction between the liposome and the target protein.

Original languageEnglish
Article number5871996
Pages (from-to)2749-2755
Number of pages7
JournalIEEE Sensors Journal
Volume11
Issue number11
DOIs
Publication statusPublished - Oct 31 2011
Externally publishedYes

Keywords

  • Biochemical analysis
  • bionanotechnology
  • biosensors
  • chemical and biological sensors
  • liposome
  • protein

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

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