Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant

P. Lorchirachoonkul, K. Yamashita, M. Noda, Toshinori Shimanouchi, H. Umakoshi, R. Kuboi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed a highly-sensitive leakage current microsensor by using 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) liposome entrapping K 4[Fe(CN)6] solution to detect existence of biomolecules especially proteins and their dynamic conditions. In this work, the addition of guanidinium hydrochloride (GuHCl) as protein denaturant successfully leads a prominent improvement in sensitivity by 129-fold of magnitude, although target protein, which is carbonic anhydrase from bovine (CAB), weakly interacts with liposome under the normal condition. Moreover, we can use it to evaluate the conformation state of CAB protein such as 'Native', 'Molten-Globule' and 'Unfold' states and we can use this leakage current microsensor to detect other protein that do not have large interaction with the liposome.

Original languageEnglish
Title of host publicationProceedings of IEEE Sensors
Pages1056-1059
Number of pages4
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: Nov 1 2010Nov 4 2010

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period11/1/1011/4/10

Fingerprint

Microsensors
Leakage currents
Liposomes
Proteins
Carbonic anhydrase
Biomolecules
Conformations
Molten materials

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Lorchirachoonkul, P., Yamashita, K., Noda, M., Shimanouchi, T., Umakoshi, H., & Kuboi, R. (2010). Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant. In Proceedings of IEEE Sensors (pp. 1056-1059). [5690730] https://doi.org/10.1109/ICSENS.2010.5690730

Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant. / Lorchirachoonkul, P.; Yamashita, K.; Noda, M.; Shimanouchi, Toshinori; Umakoshi, H.; Kuboi, R.

Proceedings of IEEE Sensors. 2010. p. 1056-1059 5690730.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lorchirachoonkul, P, Yamashita, K, Noda, M, Shimanouchi, T, Umakoshi, H & Kuboi, R 2010, Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant. in Proceedings of IEEE Sensors., 5690730, pp. 1056-1059, 9th IEEE Sensors Conference 2010, SENSORS 2010, Waikoloa, HI, United States, 11/1/10. https://doi.org/10.1109/ICSENS.2010.5690730
Lorchirachoonkul P, Yamashita K, Noda M, Shimanouchi T, Umakoshi H, Kuboi R. Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant. In Proceedings of IEEE Sensors. 2010. p. 1056-1059. 5690730 https://doi.org/10.1109/ICSENS.2010.5690730
Lorchirachoonkul, P. ; Yamashita, K. ; Noda, M. ; Shimanouchi, Toshinori ; Umakoshi, H. ; Kuboi, R. / Sensitivity enhancement of leakage current microsensor for detection of target protein by using protein denaturant. Proceedings of IEEE Sensors. 2010. pp. 1056-1059
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