Abstract
14C-acetate is preferentially taken up by astrocytes, and is a useful tool for measurement of glial metabolism. The aim of this study was to determine the effects of short-term ischemia on 14C-acetate uptake in the rat brain. The middle cerebral artery was occluded for 3, 10, or 30 minutes. Five minutes after reperfusion, rats were injected with 14C-acetate and decapitated 5 minutes later. Radioactivity concentrations in striatum and cerebral cortex were determined by autoradiography. Cerebral blood flow was also measured using 14C-iodoamphetamine. Neuronal cell death was measured by Nissl staining, and expression of monocarboxylate transporter-1 was examined by immunohistochemical staining. A significant reduction of 14C-acetate uptake was observed in striatum by 3 minutes of occlusion. The degree of reduction of 14C-acetate uptake and reduction area were increased with occlusion period. In contrast, within the same region the regional blood flow was increased by 10 minutes of occlusion, suggesting that uptake of 14C-acetate was independent on blood flow. No neural cell death was detected, and no significant alteration of monocarboxylate transporter-1 expression was observed by 30 minutes of occlusion. These results indicate that 14C-acetate uptake is a sensitive marker for glial metabolism in the ischemic rat brain.
| Original language | English |
|---|---|
| Pages (from-to) | 77-81 |
| Number of pages | 5 |
| Journal | Journal of Stroke and Cerebrovascular Diseases |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 2007 |
| Externally published | Yes |
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Keywords
- C-acetate uptake
- glia
- Ischemia
- rat
ASJC Scopus subject areas
- Clinical Neurology
- Surgery
- Health Professions(all)
- Medicine(all)
Cite this
Sensitive Reduction in 14C-acetate Uptake in a Short-term Ischemic Rat Brain. / Hosoi, Rie; Kashiwagi, Yuto; Tokumura, Miwa; Abe, Koji; Hatazawa, Jun; Inoue, Osamu.
In: Journal of Stroke and Cerebrovascular Diseases, Vol. 16, No. 2, 03.2007, p. 77-81.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sensitive Reduction in 14C-acetate Uptake in a Short-term Ischemic Rat Brain
AU - Hosoi, Rie
AU - Kashiwagi, Yuto
AU - Tokumura, Miwa
AU - Abe, Koji
AU - Hatazawa, Jun
AU - Inoue, Osamu
PY - 2007/3
Y1 - 2007/3
N2 - 14C-acetate is preferentially taken up by astrocytes, and is a useful tool for measurement of glial metabolism. The aim of this study was to determine the effects of short-term ischemia on 14C-acetate uptake in the rat brain. The middle cerebral artery was occluded for 3, 10, or 30 minutes. Five minutes after reperfusion, rats were injected with 14C-acetate and decapitated 5 minutes later. Radioactivity concentrations in striatum and cerebral cortex were determined by autoradiography. Cerebral blood flow was also measured using 14C-iodoamphetamine. Neuronal cell death was measured by Nissl staining, and expression of monocarboxylate transporter-1 was examined by immunohistochemical staining. A significant reduction of 14C-acetate uptake was observed in striatum by 3 minutes of occlusion. The degree of reduction of 14C-acetate uptake and reduction area were increased with occlusion period. In contrast, within the same region the regional blood flow was increased by 10 minutes of occlusion, suggesting that uptake of 14C-acetate was independent on blood flow. No neural cell death was detected, and no significant alteration of monocarboxylate transporter-1 expression was observed by 30 minutes of occlusion. These results indicate that 14C-acetate uptake is a sensitive marker for glial metabolism in the ischemic rat brain.
AB - 14C-acetate is preferentially taken up by astrocytes, and is a useful tool for measurement of glial metabolism. The aim of this study was to determine the effects of short-term ischemia on 14C-acetate uptake in the rat brain. The middle cerebral artery was occluded for 3, 10, or 30 minutes. Five minutes after reperfusion, rats were injected with 14C-acetate and decapitated 5 minutes later. Radioactivity concentrations in striatum and cerebral cortex were determined by autoradiography. Cerebral blood flow was also measured using 14C-iodoamphetamine. Neuronal cell death was measured by Nissl staining, and expression of monocarboxylate transporter-1 was examined by immunohistochemical staining. A significant reduction of 14C-acetate uptake was observed in striatum by 3 minutes of occlusion. The degree of reduction of 14C-acetate uptake and reduction area were increased with occlusion period. In contrast, within the same region the regional blood flow was increased by 10 minutes of occlusion, suggesting that uptake of 14C-acetate was independent on blood flow. No neural cell death was detected, and no significant alteration of monocarboxylate transporter-1 expression was observed by 30 minutes of occlusion. These results indicate that 14C-acetate uptake is a sensitive marker for glial metabolism in the ischemic rat brain.
KW - C-acetate uptake
KW - glia
KW - Ischemia
KW - rat
UR - http://www.scopus.com/inward/record.url?scp=33847646278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847646278&partnerID=8YFLogxK
U2 - 10.1016/j.jstrokecerebrovasdis.2006.11.005
DO - 10.1016/j.jstrokecerebrovasdis.2006.11.005
M3 - Article
C2 - 17689398
AN - SCOPUS:33847646278
VL - 16
SP - 77
EP - 81
JO - Journal of Stroke and Cerebrovascular Diseases
JF - Journal of Stroke and Cerebrovascular Diseases
SN - 1052-3057
IS - 2
ER -