Semliki Forest virus-based DNA expression vector: Transient protein production followed by cell death

A. Kohno, N. Emi, M. Kasai, M. Tanimoto, H. Saito

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We have constructed a novel DNA expression vector based on Semliki Forest virus (SFV). SFV produces non-structural proteins (nsPs) which replicate genomic RNA and amplify the mRNA encoding the structural proteins of SFV structural genes were replaced by a polylinker cassette to allow for insertion of heterologous DNA, was placed under the control of a cytomegalovirus immediate-early enhancer/promoter with a polyadenylation signal. Transfection of mammalian cells with this SFV-based plasmid vector, pSFV3-CMV-lacZ-pA, resulted in transient high-level expression of a β-glactosidase reporter gene.

Original languageEnglish
Pages (from-to)415-418
Number of pages4
JournalGene Therapy
Volume5
Issue number3
DOIs
Publication statusPublished - 1998

Keywords

  • Plasmid vector
  • Self-amplifying vector
  • Semliki Forest virus

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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