Abstract
Plasmodium falciparum has for some time been developing resistance against known anti-malarial drugs, and therefore a new drug is urgently needed. Selenium (Se), an essential trace element, in the form of inorganic Se, selenite (SeO32), has been reported to have an anti-plasmodial effect, but its mechanism is still unclear. In the present study, we evaluated the anti-plasmodial effect of several Se compounds against P. falciparum in vitro. The anti-plasmodial effect of several Se compounds was analysed and their apoptosis-inducing activity was evaluated by morphological observation, DNA fragmentation assay and mitochondrial function analysis. SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial effects with 50% inhibition concentration at 9, 10, 45, and 65 m, respectively, while selenate and methylselenocysteine up to 100 m have no effect on parasite growth. The effective Se compounds caused the parasites to become shrunken and pyknotic and significantly increased mitochondrial damage against P. falciparum compared to the untreated control. In conclusion, SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial activities that induce apoptosis-like cell death in P. falciparum, and the anti-plasmodial effects of Se seem to be based on its chemical forms. The apoptosis-like cell-death mechanism in P. falciparum can be beneficial to respond to the growing problem of drug resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 1852-1862 |
| Number of pages | 11 |
| Journal | Parasitology |
| Volume | 138 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2011 |
| Externally published | Yes |
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Keywords
- apoptosis
- DNA fragmentation
- drug
- malaria
- Plasmodium falciparum
- selenium
ASJC Scopus subject areas
- Parasitology
- Animal Science and Zoology
- Infectious Diseases
- Medicine(all)
Cite this
Selenium-induced apoptosis-like cell death in Plasmodium falciparum. / Suradji, Eka W.; Hatabu, Toshimitsu; Kobayashi, Kenji; Yamazaki, Chiho; Abdulah, Rizky; Nakazawa, Minato; Nakajima-Shimada, Junko; Koyama, Hiroshi.
In: Parasitology, Vol. 138, No. 14, 2011, p. 1852-1862.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selenium-induced apoptosis-like cell death in Plasmodium falciparum
AU - Suradji, Eka W.
AU - Hatabu, Toshimitsu
AU - Kobayashi, Kenji
AU - Yamazaki, Chiho
AU - Abdulah, Rizky
AU - Nakazawa, Minato
AU - Nakajima-Shimada, Junko
AU - Koyama, Hiroshi
PY - 2011
Y1 - 2011
N2 - Plasmodium falciparum has for some time been developing resistance against known anti-malarial drugs, and therefore a new drug is urgently needed. Selenium (Se), an essential trace element, in the form of inorganic Se, selenite (SeO32), has been reported to have an anti-plasmodial effect, but its mechanism is still unclear. In the present study, we evaluated the anti-plasmodial effect of several Se compounds against P. falciparum in vitro. The anti-plasmodial effect of several Se compounds was analysed and their apoptosis-inducing activity was evaluated by morphological observation, DNA fragmentation assay and mitochondrial function analysis. SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial effects with 50% inhibition concentration at 9, 10, 45, and 65 m, respectively, while selenate and methylselenocysteine up to 100 m have no effect on parasite growth. The effective Se compounds caused the parasites to become shrunken and pyknotic and significantly increased mitochondrial damage against P. falciparum compared to the untreated control. In conclusion, SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial activities that induce apoptosis-like cell death in P. falciparum, and the anti-plasmodial effects of Se seem to be based on its chemical forms. The apoptosis-like cell-death mechanism in P. falciparum can be beneficial to respond to the growing problem of drug resistance.
AB - Plasmodium falciparum has for some time been developing resistance against known anti-malarial drugs, and therefore a new drug is urgently needed. Selenium (Se), an essential trace element, in the form of inorganic Se, selenite (SeO32), has been reported to have an anti-plasmodial effect, but its mechanism is still unclear. In the present study, we evaluated the anti-plasmodial effect of several Se compounds against P. falciparum in vitro. The anti-plasmodial effect of several Se compounds was analysed and their apoptosis-inducing activity was evaluated by morphological observation, DNA fragmentation assay and mitochondrial function analysis. SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial effects with 50% inhibition concentration at 9, 10, 45, and 65 m, respectively, while selenate and methylselenocysteine up to 100 m have no effect on parasite growth. The effective Se compounds caused the parasites to become shrunken and pyknotic and significantly increased mitochondrial damage against P. falciparum compared to the untreated control. In conclusion, SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial activities that induce apoptosis-like cell death in P. falciparum, and the anti-plasmodial effects of Se seem to be based on its chemical forms. The apoptosis-like cell-death mechanism in P. falciparum can be beneficial to respond to the growing problem of drug resistance.
KW - apoptosis
KW - DNA fragmentation
KW - drug
KW - malaria
KW - Plasmodium falciparum
KW - selenium
UR - http://www.scopus.com/inward/record.url?scp=84865603794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865603794&partnerID=8YFLogxK
U2 - 10.1017/S0031182011001399
DO - 10.1017/S0031182011001399
M3 - Article
C2 - 21854677
AN - SCOPUS:84865603794
VL - 138
SP - 1852
EP - 1862
JO - Parasitology
JF - Parasitology
SN - 0031-1820
IS - 14
ER -