Selenium-induced apoptosis-like cell death in Plasmodium falciparum

Eka W. Suradji, Toshimitsu Hatabu, Kenji Kobayashi, Chiho Yamazaki, Rizky Abdulah, Minato Nakazawa, Junko Nakajima-Shimada, Hiroshi Koyama

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Plasmodium falciparum has for some time been developing resistance against known anti-malarial drugs, and therefore a new drug is urgently needed. Selenium (Se), an essential trace element, in the form of inorganic Se, selenite (SeO32), has been reported to have an anti-plasmodial effect, but its mechanism is still unclear. In the present study, we evaluated the anti-plasmodial effect of several Se compounds against P. falciparum in vitro. The anti-plasmodial effect of several Se compounds was analysed and their apoptosis-inducing activity was evaluated by morphological observation, DNA fragmentation assay and mitochondrial function analysis. SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial effects with 50% inhibition concentration at 9, 10, 45, and 65 m, respectively, while selenate and methylselenocysteine up to 100 m have no effect on parasite growth. The effective Se compounds caused the parasites to become shrunken and pyknotic and significantly increased mitochondrial damage against P. falciparum compared to the untreated control. In conclusion, SeO32, methylseleninic acid, selenomethionine and selenocystine have anti-plasmodial activities that induce apoptosis-like cell death in P. falciparum, and the anti-plasmodial effects of Se seem to be based on its chemical forms. The apoptosis-like cell-death mechanism in P. falciparum can be beneficial to respond to the growing problem of drug resistance.

Original languageEnglish
Pages (from-to)1852-1862
Number of pages11
JournalParasitology
Volume138
Issue number14
DOIs
Publication statusPublished - Dec 2011
Externally publishedYes

Keywords

  • DNA fragmentation
  • Plasmodium falciparum
  • apoptosis
  • drug
  • malaria
  • selenium

ASJC Scopus subject areas

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

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