Selective suppression of oral allergen-induced anaphylaxis by Allergin-1 on basophils in mice

Yu Hsien Lin, Satoko Tahara-Hanaoka, Kei Nagai, Soichiro Yoshikawa, Masato Kubo, Shiro Shibayama, Hajime Karasuyama, Akira Shibuya

Research output: Contribution to journalArticle

Abstract

Mast cells (MCs) play a critical role in oral allergen-induced anaphylaxis. However, the contribution of basophils to the anaphylaxis remains unclear. The inhibitory immunoreceptor Allergin-1 is highly expressed on MCs and basophils and inhibits FcϵRI-mediated signaling in MCs. Here, we show that Allergin-1-deficient (Milr1-/-) mice developed more severe hypothermia, a higher mortality rate and a greater incidence of diarrhea than did wild-type (WT) mice in an oral ovalbumin (OVA)-induced food allergy model. MC-deficient Mas-TRECK mice, which had been reconstituted with either WT or Milr1-/- bone marrow-derived cultured MCs, did not develop hypothermia in this food allergy model. On the other hand, depletion of basophils by injection of anti-CD200R3 antibody rescued Milr1-/- mice from lethal hypothermia but not from diarrhea. In vitro analyses demonstrated that Allergin-1 inhibits IgE-dependent activation of both human and mouse basophils. Thus, Allergin-1 on basophils selectively suppresses oral allergen-induced anaphylaxis.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalInternational Immunology
Volume32
Issue number3
DOIs
Publication statusPublished - Dec 3 2019
Externally publishedYes

Keywords

  • diarrhea
  • food allergy
  • mast cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Selective suppression of oral allergen-induced anaphylaxis by Allergin-1 on basophils in mice'. Together they form a unique fingerprint.

  • Cite this

    Lin, Y. H., Tahara-Hanaoka, S., Nagai, K., Yoshikawa, S., Kubo, M., Shibayama, S., Karasuyama, H., & Shibuya, A. (2019). Selective suppression of oral allergen-induced anaphylaxis by Allergin-1 on basophils in mice. International Immunology, 32(3), 213-219. https://doi.org/10.1093/intimm/dxz075