Selective separation process of proteins based on the heat stress- induced translocation across phospholipid membranes

Hiroshi Umakoshi, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Heating of several protein solutions at 40-47°C for 5-60 min in the presence of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes induced the translocation of β-galactosidase (β-gal), α-glucosidase (α- glu) and bovine carbonic anhydrase (CAB) from outer to inner aqueous phase across the liposome membrane. The translocated amounts of β-gal at various temperatures were maximized under suitable heating conditions (45°C, 30 min). Those of α-glu and CAB were maximized at 40-45 and 60°C, respectively. Each maximum value could be correlated with the corresponding local hydrophobicity of each protein evaluated by the aqueous two-phase partitioning method. The possibility to apply these heat-induced translocation phenomena to the bioseparation of proteins was successfully demonstrated for the model mixture solution of β-gal, α-glu and CAB.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume711
Issue number1-2
DOIs
Publication statusPublished - Jun 26 1998

Keywords

  • Bovine carbonic anhydrase
  • Heat stress-induced translocation
  • Phospholipid membranes
  • Proteins
  • α-Glucosidase
  • β- Galactosidase

ASJC Scopus subject areas

  • Chemistry(all)

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