Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27

Tomonori Shinya, Koji Yamaguchi, Yoshitake Desaki, Kenta Yamada, Tomoko Narisawa, Yoshihiro Kobayashi, Kanako Maeda, Maruya Suzuki, Takumi Tanimoto, Jun Takeda, Masato Nakashima, Ryota Funama, Mari Narusaka, Yoshihiro Narusaka, Hanae Kaku, Tsutomu Kawasaki, Naoto Shibuya

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Recognition of microbe-associated molecular patterns (MAMPs) initiates pattern-triggered immunity in host plants. Pattern recognition receptors (PRRs) and receptor-like cytoplasmic kinases (RLCKs) are the major components required for sensing and transduction of these molecular patterns. However, the regulation of RLCKs by PRRs and their specificity remain obscure. In this study we show that PBL27, an Arabidopsis ortholog of OsRLCK185, is an immediate downstream component of the chitin receptor CERK1 and contributes to the regulation of chitin-induced immunity in Arabidopsis. Knockout of PBL27 resulted in the suppression of several chitin-induced defense responses, including the activation of MPK3/6 and callose deposition as well as in disease resistance against fungal and bacterial infections. On the other hand, the contribution of PBL27 to flg22 signaling appears to be very limited, suggesting that PBL27 selectively regulates defense signaling downstream of specific PRR complexes. In vitro phosphorylation experiments showed that CERK1 preferentially phosphorylated PBL27 in comparison to BIK1, whereas phosphorylation of PBL27 by BAK1 was very low compared with that of BIK1. Thus, the substrate specificity of the signaling receptor-like kinases, CERK1 and BAK1, may determine the preference of downstream RLCKs.

Original languageEnglish
Pages (from-to)56-66
Number of pages11
JournalPlant Journal
Volume79
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Chitin
Cytoplasmic and Nuclear Receptors
chitin
Pattern Recognition Receptors
Arabidopsis
phosphotransferases (kinases)
Phosphotransferases
receptors
Immunity
Phosphorylation
Disease Resistance
Mycoses
Substrate Specificity
Bacterial Infections
phosphorylation
immunity
callose
substrate specificity
bacterial infections
disease resistance

Keywords

  • Arabidopsis thaliana
  • CERK1
  • chitin
  • microbe-associated molecular pattern
  • PBL27
  • plant immunity
  • receptor
  • receptor-like cytoplasmic kinase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics
  • Medicine(all)

Cite this

Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27. / Shinya, Tomonori; Yamaguchi, Koji; Desaki, Yoshitake; Yamada, Kenta; Narisawa, Tomoko; Kobayashi, Yoshihiro; Maeda, Kanako; Suzuki, Maruya; Tanimoto, Takumi; Takeda, Jun; Nakashima, Masato; Funama, Ryota; Narusaka, Mari; Narusaka, Yoshihiro; Kaku, Hanae; Kawasaki, Tsutomu; Shibuya, Naoto.

In: Plant Journal, Vol. 79, No. 1, 2014, p. 56-66.

Research output: Contribution to journalArticle

Shinya, T, Yamaguchi, K, Desaki, Y, Yamada, K, Narisawa, T, Kobayashi, Y, Maeda, K, Suzuki, M, Tanimoto, T, Takeda, J, Nakashima, M, Funama, R, Narusaka, M, Narusaka, Y, Kaku, H, Kawasaki, T & Shibuya, N 2014, 'Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27', Plant Journal, vol. 79, no. 1, pp. 56-66. https://doi.org/10.1111/tpj.12535
Shinya, Tomonori ; Yamaguchi, Koji ; Desaki, Yoshitake ; Yamada, Kenta ; Narisawa, Tomoko ; Kobayashi, Yoshihiro ; Maeda, Kanako ; Suzuki, Maruya ; Tanimoto, Takumi ; Takeda, Jun ; Nakashima, Masato ; Funama, Ryota ; Narusaka, Mari ; Narusaka, Yoshihiro ; Kaku, Hanae ; Kawasaki, Tsutomu ; Shibuya, Naoto. / Selective regulation of the chitin-induced defense response by the Arabidopsis receptor-like cytoplasmic kinase PBL27. In: Plant Journal. 2014 ; Vol. 79, No. 1. pp. 56-66.
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AU - Narisawa, Tomoko

AU - Kobayashi, Yoshihiro

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AU - Suzuki, Maruya

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AU - Nakashima, Masato

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