Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite

Eiji Fujii, Mizuki Ohkubo, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka, Koji Kawabata, Christian Bonhomme, Florence Babonneau

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β2-microglobulin (β2-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to β2-MG.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalActa Biomaterialia
Volume2
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Durapatite
Hydroxyapatite
Adsorption
Zinc
Bovine Serum Albumin
Crystalline materials
Proteins
Specific surface area
Transmission Electron Microscopy
Crystallites
X-Ray Diffraction
X ray diffraction analysis
Observation
Transmission electron microscopy

Keywords

  • Adsorption
  • Albumin
  • Calcium phosphate
  • Hydroxyapatite
  • Zinc

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite. / Fujii, Eiji; Ohkubo, Mizuki; Tsuru, Kanji; Hayakawa, Satoshi; Osaka, Akiyoshi; Kawabata, Koji; Bonhomme, Christian; Babonneau, Florence.

In: Acta Biomaterialia, Vol. 2, No. 1, 01.2006, p. 69-74.

Research output: Contribution to journalArticle

Fujii, Eiji ; Ohkubo, Mizuki ; Tsuru, Kanji ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Kawabata, Koji ; Bonhomme, Christian ; Babonneau, Florence. / Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite. In: Acta Biomaterialia. 2006 ; Vol. 2, No. 1. pp. 69-74.
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AU - Bonhomme, Christian

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