Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite

Eiji Fujii; Mizuki Ohkubo; Kanji Tsuru; Satoshi Hayakawa; Akiyoshi Osaka; Koji Kawabata; Christian Bonhomme; Florence Babonneau

doi:10.1016/j.actbio.2005.09.002

Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite

Eiji Fujii, Mizuki Ohkubo, Kanji Tsuru, Satoshi Hayakawa, Akiyoshi Osaka, Koji Kawabata, Christian Bonhomme, Florence Babonneau

Research output: Contribution to journal › Article › peer-review

147 Citations (Scopus)

Abstract

Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β₂-microglobulin (β₂-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to β₂-MG.

Original language	English
Pages (from-to)	69-74
Number of pages	6
Journal	Acta Biomaterialia
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.actbio.2005.09.002
Publication status	Published - Jan 2006

Keywords

Adsorption
Albumin
Calcium phosphate
Hydroxyapatite
Zinc

ASJC Scopus subject areas

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

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Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite. / Fujii, Eiji; Ohkubo, Mizuki; Tsuru, Kanji; Hayakawa, Satoshi; Osaka, Akiyoshi; Kawabata, Koji; Bonhomme, Christian; Babonneau, Florence.

In: Acta Biomaterialia, Vol. 2, No. 1, 01.2006, p. 69-74.

Research output: Contribution to journal › Article › peer-review

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title = "Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite",

abstract = "Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β2-microglobulin (β2-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to β2-MG.",

keywords = "Adsorption, Albumin, Calcium phosphate, Hydroxyapatite, Zinc",

author = "Eiji Fujii and Mizuki Ohkubo and Kanji Tsuru and Satoshi Hayakawa and Akiyoshi Osaka and Koji Kawabata and Christian Bonhomme and Florence Babonneau",

note = "Funding Information: This study was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No.16360330, Z1416005) and The Hosokawa Powder Technology Foundation.",

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AU - Fujii, Eiji

AU - Ohkubo, Mizuki

AU - Tsuru, Kanji

AU - Hayakawa, Satoshi

AU - Osaka, Akiyoshi

AU - Kawabata, Koji

AU - Bonhomme, Christian

AU - Babonneau, Florence

N1 - Funding Information: This study was supported by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No.16360330, Z1416005) and The Hosokawa Powder Technology Foundation.

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N2 - Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β2-microglobulin (β2-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to β2-MG.

AB - Nano-crystalline Zn-containing hydroxyapatite (ZnHAp) was prepared by the wet-chemical method and the selective adsorption of essential proteins was examined, taking bovine serum albumin (BSA) and pathogenic protein such as β2-microglobulin (β2-MG) as model proteins. Transmission electron microscopy observation and X-ray diffraction analysis indicated that the increase of Zn content led to smaller crystallites and their specific surface area of ZnHAps increased with increasing Zn content, accordingly. Furthermore, the amounts of BSA adsorption on ZnHAp particles decreased with increasing Zn content in spite of the increase in the specific surface area. It is thus concluded that nano-crystalline ZnHAps had a highly selective adsorption property with regard to β2-MG.

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KW - Calcium phosphate

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