Selective Inhibition of the 12-Lipoxygenase Pathway of Arachidonic Acid Metabolism by L-Arginine or Sodium Nitroprusside in Intact Human Platelets

Mikiya Nakatsuka, Yoichi Osawa

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50 Citations (Scopus)


L-arginine (1-100 μM) or sodium nitroprusside (1-100 μM) caused a concentration dependent decrease in the metabolism of exogenously added arachidonic acid via the 12-lipoxygenase pathway in intact human platelets, as determined by the use of an HPLC assay. NG-monomethyl-L-arginine, but not the D-isomer of this compound, diminished the inhibitory effect of L-arginine. The D isomer of arginine had no effect. The cyclooxygenase pathway was much less susceptible to these effects. This study indicated that nitric oxide formed in intact human platelets selectively inhibited 12-lipoxygenase over that of cyclooxygenase and suggests that such inhibition may be an important regulatory mechanism.

Original languageEnglish
Pages (from-to)1630-1634
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - Jan 1 1994
Externally publishedYes


ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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