Selective extraction of active α-chymotrypsin by reversed micelles

Masahiro Goto, Maki Korenaga, Tsutomu Ono, Shintaro Furusaki

Research output: Contribution to journalArticle

Abstract

Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed.

Original languageEnglish
Pages (from-to)184-185
Number of pages2
JournalKagaku Kogaku Ronbunshu
Volume27
Issue number2
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Chymotrypsin
Micelles
Phosphoric acid
Proteins
Anionic surfactants
Structural properties
dioleoyl phosphate

Keywords

  • AOT
  • Extraction
  • Protein separation
  • Reverse micelle
  • Surfactant

ASJC Scopus subject areas

  • Materials Science (miscellaneous)

Cite this

Goto, M., Korenaga, M., Ono, T., & Furusaki, S. (2001). Selective extraction of active α-chymotrypsin by reversed micelles. Kagaku Kogaku Ronbunshu, 27(2), 184-185.

Selective extraction of active α-chymotrypsin by reversed micelles. / Goto, Masahiro; Korenaga, Maki; Ono, Tsutomu; Furusaki, Shintaro.

In: Kagaku Kogaku Ronbunshu, Vol. 27, No. 2, 2001, p. 184-185.

Research output: Contribution to journalArticle

Goto, M, Korenaga, M, Ono, T & Furusaki, S 2001, 'Selective extraction of active α-chymotrypsin by reversed micelles', Kagaku Kogaku Ronbunshu, vol. 27, no. 2, pp. 184-185.
Goto, Masahiro ; Korenaga, Maki ; Ono, Tsutomu ; Furusaki, Shintaro. / Selective extraction of active α-chymotrypsin by reversed micelles. In: Kagaku Kogaku Ronbunshu. 2001 ; Vol. 27, No. 2. pp. 184-185.
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