Selective Extraction of Active α-Chymotrypsin by Reversed Micelles

Masahiro Goto; Maki Korenaga; Tsutomu Ono; Shintaro Furusaki

doi:10.1252/kakoronbunshu.27.181

Selective Extraction of Active α-Chymotrypsin by Reversed Micelles

Masahiro Goto, Maki Korenaga, Tsutomu Ono, Shintaro Furusaki

Research output: Contribution to journal › Article

Abstract

Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed. Key words: extraction, reverse micelle, surfactant, protein separation, AOT.

Original language	English
Pages (from-to)	181-185
Number of pages	5
Journal	KAGAKU KOGAKU RONBUNSHU
Volume	27
Issue number	2
DOIs	https://doi.org/10.1252/kakoronbunshu.27.181
Publication status	Published - Jan 1 2001
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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Goto, M., Korenaga, M., Ono, T., & Furusaki, S. (2001). Selective Extraction of Active α-Chymotrypsin by Reversed Micelles. KAGAKU KOGAKU RONBUNSHU, 27(2), 181-185. https://doi.org/10.1252/kakoronbunshu.27.181

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