Abstract
Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 184-185 |
| Number of pages | 2 |
| Journal | Kagaku Kogaku Ronbunshu |
| Volume | 27 |
| Issue number | 2 |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- AOT
- Extraction
- Protein separation
- Reverse micelle
- Surfactant
ASJC Scopus subject areas
- Materials Science (miscellaneous)
Cite this
Goto, M., Korenaga, M., Ono, T., & Furusaki, S. (2001). Selective extraction of active α-chymotrypsin by reversed micelles. Kagaku Kogaku Ronbunshu, 27(2), 184-185.