Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed.
|Number of pages||2|
|Journal||Kagaku Kogaku Ronbunshu|
|Publication status||Published - 2001|
- Protein separation
- Reverse micelle
ASJC Scopus subject areas
- Materials Science (miscellaneous)