Abstract
Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 184-185 |
| Number of pages | 2 |
| Journal | Kagaku Kogaku Ronbunshu |
| Volume | 27 |
| Issue number | 2 |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- AOT
- Extraction
- Protein separation
- Reverse micelle
- Surfactant
ASJC Scopus subject areas
- Materials Science (miscellaneous)
Cite this
Selective extraction of active α-chymotrypsin by reversed micelles. / Goto, Masahiro; Korenaga, Maki; Ono, Tsutomu; Furusaki, Shintaro.
In: Kagaku Kogaku Ronbunshu, Vol. 27, No. 2, 2001, p. 184-185.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selective extraction of active α-chymotrypsin by reversed micelles
AU - Goto, Masahiro
AU - Korenaga, Maki
AU - Ono, Tsutomu
AU - Furusaki, Shintaro
PY - 2001
Y1 - 2001
N2 - Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed.
AB - Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed.
KW - AOT
KW - Extraction
KW - Protein separation
KW - Reverse micelle
KW - Surfactant
UR - http://www.scopus.com/inward/record.url?scp=24044532926&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24044532926&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:24044532926
VL - 27
SP - 184
EP - 185
JO - Kagaku Kogaku Ronbunshu
JF - Kagaku Kogaku Ronbunshu
SN - 0386-216X
IS - 2
ER -