TY - JOUR
T1 - Selective Extraction of Active α-Chymotrypsin by Reversed Micelles
AU - Goto, Masahiro
AU - Korenaga, Maki
AU - Ono, Tsutomu
AU - Furusaki, Shintaro
PY - 2001
Y1 - 2001
N2 - Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed. Key words: extraction, reverse micelle, surfactant, protein separation, AOT.
AB - Extraction behavior of active and denatured α-chymotrypsin is investigated by the reversed micellar extraction technique. The anionic surfactant dioleyl phosphoric acid (DOLPA) selectively extracted the active protein over the denatured protein. However, the reversed micelles using AOT do not afford selectivity. Based on the difference in the structural properties between the active and the heated α-chymotrypsin, the extraction behavior by the DOLPA reversed micelles is discussed. Key words: extraction, reverse micelle, surfactant, protein separation, AOT.
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U2 - 10.1252/kakoronbunshu.27.181
DO - 10.1252/kakoronbunshu.27.181
M3 - Article
AN - SCOPUS:24044532926
VL - 27
SP - 181
EP - 185
JO - Kagaku Kogaku Ronbunshu
JF - Kagaku Kogaku Ronbunshu
SN - 0386-216X
IS - 2
ER -