Selective chemical treatment of cellular microdomains using multiple laminar streams

Shuichi Takayama, Emanuele Ostuni, Philip LeDuc, Keiji Naruse, Donald E. Ingber, George M. Whitesides

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

There are many experiments in which it would be useful to treat a part of the surface or interior of a cell with a biochemical reagent. It is difficult, however, to achieve subcellular specificity, because small molecules diffuse distances equal to the extent of the cell in seconds. This paper demonstrates experimentally, and analyzes theoretically, the use of multiple laminar fluid streams in microfluidic channels to deliver reagents to, and remove them from, cells with subcellular spatial selectivity. The technique made it possible to label different subpopulations of mitochondria fluorescently, to disrupt selected regions of the cytoskeleton chemically, to dislodge limited areas of cell-substrate adhesions enzymatically, and to observe microcompartmental endocytosis within individual cells. This technique does not require microinjection or immobilization of reagents onto nondiffusive objects; it opens a new window into cell biology.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalChemistry and Biology
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 1 2003
Externally publishedYes

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Cytology
Mitochondria
Microfluidics
Labels
Microinjections
Therapeutics
Adhesion
Endocytosis
Cytoskeleton
Cell Adhesion
Immobilization
Cell Biology
Molecules
Fluids
Substrates
Experiments

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Selective chemical treatment of cellular microdomains using multiple laminar streams. / Takayama, Shuichi; Ostuni, Emanuele; LeDuc, Philip; Naruse, Keiji; Ingber, Donald E.; Whitesides, George M.

In: Chemistry and Biology, Vol. 10, No. 2, 01.02.2003, p. 123-130.

Research output: Contribution to journalArticle

Takayama, Shuichi ; Ostuni, Emanuele ; LeDuc, Philip ; Naruse, Keiji ; Ingber, Donald E. ; Whitesides, George M. / Selective chemical treatment of cellular microdomains using multiple laminar streams. In: Chemistry and Biology. 2003 ; Vol. 10, No. 2. pp. 123-130.
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