Secretory production system of bionanocapsules using a stably transfected insect cell line

Takuya Shishido, Masaru Muraoka, Masakazu Ueda, Masaharu Seno, Katsuyuki Tanizawa, Shun'ichi Kuroda, Hideki Fukuda, Akihiko Kondo

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bionanocapsules (BNCs) are hollow nanoscale particles composed of L protein of the hepatitis B virus surface antigen that represent specific affinity for human hepatocytes. BNCs can transfer genes and drugs into human hepatocytes efficiently and specifically. BNC can be expressed in yeast cells. In this study, we developed a new L particle production system using a stably transfected insect cell line. For this purpose, we established a host-vector system using the Trichoplusia ni insect cell line. L particles were efficiently secreted by the overexpression of the L protein, which was fused to the secretion signal peptide. The concentration of L particles was reached approximately 1.7 μg/ml in 5 days during cultivation in a serum-free medium without antibiotic selective pressure. The production of L particles was maintained for at least 75 days. The secretory production of L particles facilitated their easy purification by chromatography. Furthermore, it was demonstrated that purified L particles can transfect only human hepatocytes. Therefore, an insect cell expression system is an attractive tool for the production of BNC.

Original languageEnglish
Pages (from-to)505-511
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume73
Issue number3
DOIs
Publication statusPublished - Dec 2006

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Insects
Hepatocytes
Cells
Cell Line
Serum-Free Culture Media
Hepatitis B Surface Antigens
Protein Sorting Signals
Proteins
Gene transfer
Chromatography
Antibiotics
Surface Antigens
Antigens
Yeasts
Viruses
Yeast
Anti-Bacterial Agents
Purification
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

Secretory production system of bionanocapsules using a stably transfected insect cell line. / Shishido, Takuya; Muraoka, Masaru; Ueda, Masakazu; Seno, Masaharu; Tanizawa, Katsuyuki; Kuroda, Shun'ichi; Fukuda, Hideki; Kondo, Akihiko.

In: Applied Microbiology and Biotechnology, Vol. 73, No. 3, 12.2006, p. 505-511.

Research output: Contribution to journalArticle

Shishido, T, Muraoka, M, Ueda, M, Seno, M, Tanizawa, K, Kuroda, S, Fukuda, H & Kondo, A 2006, 'Secretory production system of bionanocapsules using a stably transfected insect cell line', Applied Microbiology and Biotechnology, vol. 73, no. 3, pp. 505-511. https://doi.org/10.1007/s00253-006-0486-3
Shishido, Takuya ; Muraoka, Masaru ; Ueda, Masakazu ; Seno, Masaharu ; Tanizawa, Katsuyuki ; Kuroda, Shun'ichi ; Fukuda, Hideki ; Kondo, Akihiko. / Secretory production system of bionanocapsules using a stably transfected insect cell line. In: Applied Microbiology and Biotechnology. 2006 ; Vol. 73, No. 3. pp. 505-511.
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