Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells

Teruko Nishio, Masato Maruyama, Toyokazu Yoshida, Yoshihiko Watanabe, Makiya Nishikawa, Yoshinobu Takakura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.

Original languageEnglish
Pages (from-to)1653-1655
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number10
DOIs
Publication statusPublished - Oct 1 2004
Externally publishedYes

Keywords

  • Epithelial cell
  • Interferon
  • LLC-PK
  • Polarity
  • Secretory protein
  • μ1B

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Fingerprint Dive into the research topics of 'Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK<sub>1</sub> cells'. Together they form a unique fingerprint.

  • Cite this