Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells

Teruko Nishio, Masato Maruyama, Toyokazu Yoshida, Yoshihiko Watanabe, Makiya Nishikawa, Yoshinobu Takakura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.

Original languageEnglish
Pages (from-to)1653-1655
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number10
DOIs
Publication statusPublished - Oct 1 2004
Externally publishedYes

Fingerprint

LLC-PK1 Cells
Interferons
Swine
Epithelial Cells
Kidney
trans-Golgi Network
Proximal Kidney Tubule
Transfection
Cell Line
Proteins

Keywords

  • μ1B
  • Epithelial cell
  • Interferon
  • LLC-PK
  • Polarity
  • Secretory protein

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells. / Nishio, Teruko; Maruyama, Masato; Yoshida, Toyokazu; Watanabe, Yoshihiko; Nishikawa, Makiya; Takakura, Yoshinobu.

In: Biological and Pharmaceutical Bulletin, Vol. 27, No. 10, 01.10.2004, p. 1653-1655.

Research output: Contribution to journalArticle

Nishio, Teruko ; Maruyama, Masato ; Yoshida, Toyokazu ; Watanabe, Yoshihiko ; Nishikawa, Makiya ; Takakura, Yoshinobu. / Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells. In: Biological and Pharmaceutical Bulletin. 2004 ; Vol. 27, No. 10. pp. 1653-1655.
@article{4a0937ab2c614322b027dc43aac7dae2,
title = "Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells",
abstract = "We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.",
keywords = "μ1B, Epithelial cell, Interferon, LLC-PK, Polarity, Secretory protein",
author = "Teruko Nishio and Masato Maruyama and Toyokazu Yoshida and Yoshihiko Watanabe and Makiya Nishikawa and Yoshinobu Takakura",
year = "2004",
month = "10",
day = "1",
doi = "10.1248/bpb.27.1653",
language = "English",
volume = "27",
pages = "1653--1655",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "10",

}

TY - JOUR

T1 - Secretion mode and subcellular localization of human interferon-β exogenously expressed in porcine renal epithelial LLC-PK1 cells

AU - Nishio, Teruko

AU - Maruyama, Masato

AU - Yoshida, Toyokazu

AU - Watanabe, Yoshihiko

AU - Nishikawa, Makiya

AU - Takakura, Yoshinobu

PY - 2004/10/1

Y1 - 2004/10/1

N2 - We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.

AB - We have investigated the secretion polarity of human interferon-β (HuIFN-β) exogenously expressed in the porcine renal proximal tubule cell line, LLC-PK1. In these cells, stably expressed HuIFN-β was secreted to the apical and basolateral sides. However, when transiently expressed by apical lipofection, HuIFN-β was secreted to both cell sides, while basolateral-preferential secretion was seen for basal transfection. Confocal imaging using enhanced green fluorescent protein (EGFP)-tagged HuIFN-β revealed no difference in the subcellular distribution in either of the chimeric protein-expressing cells examined. These results suggest that the secretion polarity of HuIFN-β is regulated by a post trans-Golgi network in a cell type-dependent manner.

KW - μ1B

KW - Epithelial cell

KW - Interferon

KW - LLC-PK

KW - Polarity

KW - Secretory protein

UR - http://www.scopus.com/inward/record.url?scp=21644450519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21644450519&partnerID=8YFLogxK

U2 - 10.1248/bpb.27.1653

DO - 10.1248/bpb.27.1653

M3 - Article

C2 - 15467213

AN - SCOPUS:21644450519

VL - 27

SP - 1653

EP - 1655

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 10

ER -