Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma

Gulsan Ara Sathi, Miho Inoue, Hidemitsu Harada, Andrea P. Rodriguez, Ryo Tamamura, Hidetsugu Tsujigiwa, Silvia S. Borkosky, Mehmet Gunduz, Hitoshi Nagatsuka

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23 Citations (Scopus)

Abstract

Secreted frizzled related protein (sFRP)-2, a Wnt antagonist, was strongly expressed by both stromal and tumor cells of ameloblastoma. The aim of this study is to evaluate whether sFRP-2 secreted from tumor cells have any direct role in suppressed bone formation or not. A pre-osteoblastic cell line, KUSA/A1 cells, cultured in conditioned medium of an ameloblastoma-derived cell line (AM-1CM) was used in the study. Alkaline phosphatase (ALP) activity, alizarin red staining, mineral quantification and MTS assay was performed. Wnt-canonical pathway is a major pathway for osteoblasts. Antagonists of this pathway, sFRP-1, 2 and 3, were detected by immunohistochemistry and western blot analysis. KUSA/A1 cells cultured in AM-1CM showed high cell proliferation, low ALP activity without mineralized matrix deposition. sFRP-2 was strongly expressed in ameloblastoma tissue and AM-1 cells. After sFRP-2 depletion, the cells showed diffuse mineralization. In this study, it was confirmed that ameloblastoma cells have a major role in decreased bone formation by secreting sFRP-2 in cell culture model. Though, sFRP-2 has great effect on tumor progression, inhibition of sFRP-2's anti-bone formation activity and cell proliferative activity may reduce the invasive property of ameloblastoma and possibility of recurrence rate.

Original languageEnglish
Pages (from-to)856-860
Number of pages5
JournalOral Oncology
Volume45
Issue number10
DOIs
Publication statusPublished - Oct 1 2009

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Keywords

  • AM-1 cells
  • Amelolastoma
  • KUSA/A1 cells
  • Mineralized bone matrix
  • Wnt-canonical pathway
  • sFRP-2

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

Cite this

Sathi, G. A., Inoue, M., Harada, H., Rodriguez, A. P., Tamamura, R., Tsujigiwa, H., Borkosky, S. S., Gunduz, M., & Nagatsuka, H. (2009). Secreted frizzled related protein (sFRP)-2 inhibits bone formation and promotes cell proliferation in ameloblastoma. Oral Oncology, 45(10), 856-860. https://doi.org/10.1016/j.oraloncology.2009.02.001