Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer

S. A. Tahir, G. Yang, S. Ebara, T. L. Timme, T. Satoh, L. Li, A. Goltsov, M. Ittmann, J. D. Morrisett, T. C. Thompson

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Caveolin-1 is an integral protein of caveolae, known to play important roles in signal transduction and lipid transport. We demonstrate that caveolin-1 expression is significantly increased in primary and metastatic human prostate cancer after androgen ablation therapy. We also show that caveolin-1 is secreted by androgen-insensitive prostate cancer cells, and that this secretion is regulated by steroid hormones. Significantly, caveolin-1 was detected in the MDL3 fraction of serum specimens from patients with advanced prostate cancer and to a lesser extent in normal subjects. Conditioned media from high passage caveolin-1 secreting, androgen-insensitive, LNCaP cells stimulated increased viability and clonal growth of low passage, caveolin-1-negative, androgen-sensitive, LNCaP cells in vitro, and this effect was blocked by treating the media with caveolin-1 antibody. i.p. injections of caveolin-1 antibody suppressed the orthotopic growth and spontaneous metastasis of highly metastatic, androgen-insensitive caveolin-1-secreting mouse prostate cancer. Overall, our results establish caveolin-1 as an autocrine/paracrine factor that is associated with androgen-insensitive prostate cancer. We demonstrate the potential for caveolin-1 as a therapeutic target for this important malignancy.

Original languageEnglish
Pages (from-to)3882-3885
Number of pages4
JournalCancer Research
Volume61
Issue number10
Publication statusPublished - May 15 2001
Externally publishedYes

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Caveolin 1
Androgens
Prostatic Neoplasms
Cell Survival
Neoplasm Metastasis
Growth
Caveolae
Antibodies
Conditioned Culture Medium
Signal Transduction
Steroids

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tahir, S. A., Yang, G., Ebara, S., Timme, T. L., Satoh, T., Li, L., ... Thompson, T. C. (2001). Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer. Cancer Research, 61(10), 3882-3885.

Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer. / Tahir, S. A.; Yang, G.; Ebara, S.; Timme, T. L.; Satoh, T.; Li, L.; Goltsov, A.; Ittmann, M.; Morrisett, J. D.; Thompson, T. C.

In: Cancer Research, Vol. 61, No. 10, 15.05.2001, p. 3882-3885.

Research output: Contribution to journalArticle

Tahir, SA, Yang, G, Ebara, S, Timme, TL, Satoh, T, Li, L, Goltsov, A, Ittmann, M, Morrisett, JD & Thompson, TC 2001, 'Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer', Cancer Research, vol. 61, no. 10, pp. 3882-3885.
Tahir, S. A. ; Yang, G. ; Ebara, S. ; Timme, T. L. ; Satoh, T. ; Li, L. ; Goltsov, A. ; Ittmann, M. ; Morrisett, J. D. ; Thompson, T. C. / Secreted caveolin-1 stimulates cell survival/clonal growth and contributes to metastasis in androgen-insensitive prostate cancer. In: Cancer Research. 2001 ; Vol. 61, No. 10. pp. 3882-3885.
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