SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana

Akinori Kiba, Ivan Galis, Yuko Hojo, Kouhei Ohnishi, Hirofumi Yoshioka, Yasufumi Hikichi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We previously identified a gene related to the SEC14-gene phospholipid transfer protein superfamily that is induced in Nicotiana benthamiana (NbSEC14) in response to infection with Ralstonia solanacearum. We here report that NbSEC14 plays a role in plant immune responses via phospholipid-turnover. NbSEC14-silencing compromised expression of defense-related PR-4 and accumulation of jasmonic acid (JA) and its derivative JA-Ile. Transient expression of NbSEC14 induced PR-4 gene expression. Activities of diacylglycerol kinase, phospholipase C and D, and the synthesis of diacylglycerol and phosphatidic acid elicited by avirulent R. solanacearum were reduced in NbSEC14-silenced plants. Accumulation of signaling lipids and activation of diacylglycerol kinase and phospholipases were enhanced by transient expression of NbSEC14. These results suggest that the NbSEC14 protein plays a role at the interface between lipid signaling-metabolism and plant innate immune responses.

Original languageEnglish
Article numbere98150
JournalPLoS One
Volume9
Issue number5
DOIs
Publication statusPublished - May 20 2014

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Ralstonia solanacearum
Plant Immunity
Diacylglycerol Kinase
Phospholipid Transfer Proteins
Nicotiana benthamiana
diacylglycerol kinase
Tobacco
Genes
immune response
jasmonic acid
Lipids
Phospholipase D
Phosphatidic Acids
Phospholipases
Diglycerides
Type C Phospholipases
lipids
Lipid Metabolism
Innate Immunity
Metabolism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana. / Kiba, Akinori; Galis, Ivan; Hojo, Yuko; Ohnishi, Kouhei; Yoshioka, Hirofumi; Hikichi, Yasufumi.

In: PLoS One, Vol. 9, No. 5, e98150, 20.05.2014.

Research output: Contribution to journalArticle

Kiba, Akinori ; Galis, Ivan ; Hojo, Yuko ; Ohnishi, Kouhei ; Yoshioka, Hirofumi ; Hikichi, Yasufumi. / SEC14 phospholipid transfer protein is involved in lipid signaling-mediated plant immune responses in Nicotiana benthamiana. In: PLoS One. 2014 ; Vol. 9, No. 5.
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