TY - JOUR
T1 - Seawater transfer down-regulates C-type natriuretic peptide-3 expression in prolactin-producing cells of Japanese eel
T2 - Negative correlation with plasma chloride concentration
AU - Katayama, Yukitoshi
AU - Wong, Marty Kwok Shing
AU - Kusakabe, Makoto
AU - Fujio, Megumi
AU - Takahashi, Natsuki
AU - Yaguchi, Miku
AU - Tsukada, Takehiro
N1 - Funding Information:
This work was supported in part by JSPS KAKENHI Grants (Numbers 16 K18575 to MW), the Scientific research funds to TT from the Faculty of Science at Toho University.We thank Ms. Sanae Ogasawara and Prof. Susumu Hyodo of Atmosphere and Ocean Research Institute, the University of Tokyo for their support in ions and osmolality measurement, Dr. Wataru Isawasaki of the University of Tokyo for providing eel genome database, and Dr. Christopher A. Loretz of State University of New York at Buffalo for critical reading of the manuscript.
Funding Information:
This work was supported in part by JSPS KAKENHI Grants (Numbers 16 K18575 to MW), the Scientific research funds to TT from the Faculty of Science at Toho University .
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - In euryhaline fishes, atrial and B-type natriuretic peptides are important hormones in hypo-osmoregulation, whereas osmoregulatory functions of C-type natriuretic peptides (CNPs) remain to be investigated. Although four CNP isoforms (CNP1-4) are mainly expressed in the brain, multiorgan expression of CNP3 was found in euryhaline Japanese eel, Anguilla japonica. Here we identified the CNP3-expressing cells and examined their response to osmotic stress in eel. CNP3 was expressed in several endocrine cells: prolactin-producing cells (pituitary), glucagon-producing cells (pancreas), and cardiomyocytes (heart). Pituitary CNP3 expression was the highest among organs and was decreased following seawater transfer, followed by a decrease in the freshwater-adaptating (hyper-osmoregulatory) hormone prolactin. We also showed the negative correlation between CNP3/prolactin expression in the pituitary and plasma Cl− concentration, but not for plasma Na+ concentration. These results suggest that CNP3 in the pituitary (and pancreas) plays a critical role in freshwater adaptation of euryhaline eel together with prolactin.
AB - In euryhaline fishes, atrial and B-type natriuretic peptides are important hormones in hypo-osmoregulation, whereas osmoregulatory functions of C-type natriuretic peptides (CNPs) remain to be investigated. Although four CNP isoforms (CNP1-4) are mainly expressed in the brain, multiorgan expression of CNP3 was found in euryhaline Japanese eel, Anguilla japonica. Here we identified the CNP3-expressing cells and examined their response to osmotic stress in eel. CNP3 was expressed in several endocrine cells: prolactin-producing cells (pituitary), glucagon-producing cells (pancreas), and cardiomyocytes (heart). Pituitary CNP3 expression was the highest among organs and was decreased following seawater transfer, followed by a decrease in the freshwater-adaptating (hyper-osmoregulatory) hormone prolactin. We also showed the negative correlation between CNP3/prolactin expression in the pituitary and plasma Cl− concentration, but not for plasma Na+ concentration. These results suggest that CNP3 in the pituitary (and pancreas) plays a critical role in freshwater adaptation of euryhaline eel together with prolactin.
KW - CNP
KW - Euryhaline fish
KW - Freshwater adaptation
KW - Osmoregulation
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U2 - 10.1016/j.mce.2020.110780
DO - 10.1016/j.mce.2020.110780
M3 - Article
C2 - 32142860
AN - SCOPUS:85080991818
VL - 507
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
M1 - 110780
ER -