Abstract
Several reports have been published on blood leptin concentrations in feral animals, including members of the Carnivora, using a commercially available multi-species radioimmunoassay (RIA) kit with anti-human leptin antibody. However, we observed weak immunoreactivity between recombinant canine leptin and anti-human leptin antibody, suggesting a limitation in the applicability of the RIA kit for leptin assays in Carnivora species. We tested the applicability of RIA and sandwich enzyme-linked immunosorbent assay (ELISA) with anti-canine leptin antibody to assay blood leptin in the dog (Canis familiaris) and the raccoon (Procyon lotor). When RIA was used for recombinant canine leptin and dog sera, values were much lower than those determined by ELISA at higher concentrations (> 10 ng/ml), while rather higher at lower concentrations (<2 ng/ml). A similar discrepancy between the two methods was found for serum leptin concentrations in raccoons. Clear seasonal variations were observed by ELISA, but not by RIA, with high values in autumn (3.46 ± 0.45 ng/ml) and low values in spring and summer (0.71 ± 0.07 ng/ml). Serum leptin concentrations in raccoons correlated positively with their body weight (r = 0.753) and body mass index (r = 0.755), corroborating our previous findings of a strong positive correlation between serum leptin concentrations and body fat content in dogs. Thus, the canine leptin ELISA is useful for assays of dog and raccoon leptin, and blood leptin is a good marker of nutritional condition in the species of Carnivora assayed in this study.
| Original language | English |
|---|---|
| Pages (from-to) | 527-533 |
| Number of pages | 7 |
| Journal | Journal of Experimental Zoology Part A: Comparative Experimental Biology |
| Volume | 303 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 1 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA. / Shibata, Haruki; Akahane, Rie; Honjoh, Tsutomu; Asano, Makoto; Mominoki, Katsumi; Fujii, Kei; Suzuki, Masatsugu; Ohtaishi, Noriyuki; Ishioka, Katsumi; Ahmed, Mohamed; Soliman, Mohamed; Kimura, Kazuhiro; Saito, Masayuki.
In: Journal of Experimental Zoology Part A: Comparative Experimental Biology, Vol. 303, No. 7, 01.07.2005, p. 527-533.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA
AU - Shibata, Haruki
AU - Akahane, Rie
AU - Honjoh, Tsutomu
AU - Asano, Makoto
AU - Mominoki, Katsumi
AU - Fujii, Kei
AU - Suzuki, Masatsugu
AU - Ohtaishi, Noriyuki
AU - Ishioka, Katsumi
AU - Ahmed, Mohamed
AU - Soliman, Mohamed
AU - Kimura, Kazuhiro
AU - Saito, Masayuki
PY - 2005/7/1
Y1 - 2005/7/1
N2 - Several reports have been published on blood leptin concentrations in feral animals, including members of the Carnivora, using a commercially available multi-species radioimmunoassay (RIA) kit with anti-human leptin antibody. However, we observed weak immunoreactivity between recombinant canine leptin and anti-human leptin antibody, suggesting a limitation in the applicability of the RIA kit for leptin assays in Carnivora species. We tested the applicability of RIA and sandwich enzyme-linked immunosorbent assay (ELISA) with anti-canine leptin antibody to assay blood leptin in the dog (Canis familiaris) and the raccoon (Procyon lotor). When RIA was used for recombinant canine leptin and dog sera, values were much lower than those determined by ELISA at higher concentrations (> 10 ng/ml), while rather higher at lower concentrations (<2 ng/ml). A similar discrepancy between the two methods was found for serum leptin concentrations in raccoons. Clear seasonal variations were observed by ELISA, but not by RIA, with high values in autumn (3.46 ± 0.45 ng/ml) and low values in spring and summer (0.71 ± 0.07 ng/ml). Serum leptin concentrations in raccoons correlated positively with their body weight (r = 0.753) and body mass index (r = 0.755), corroborating our previous findings of a strong positive correlation between serum leptin concentrations and body fat content in dogs. Thus, the canine leptin ELISA is useful for assays of dog and raccoon leptin, and blood leptin is a good marker of nutritional condition in the species of Carnivora assayed in this study.
AB - Several reports have been published on blood leptin concentrations in feral animals, including members of the Carnivora, using a commercially available multi-species radioimmunoassay (RIA) kit with anti-human leptin antibody. However, we observed weak immunoreactivity between recombinant canine leptin and anti-human leptin antibody, suggesting a limitation in the applicability of the RIA kit for leptin assays in Carnivora species. We tested the applicability of RIA and sandwich enzyme-linked immunosorbent assay (ELISA) with anti-canine leptin antibody to assay blood leptin in the dog (Canis familiaris) and the raccoon (Procyon lotor). When RIA was used for recombinant canine leptin and dog sera, values were much lower than those determined by ELISA at higher concentrations (> 10 ng/ml), while rather higher at lower concentrations (<2 ng/ml). A similar discrepancy between the two methods was found for serum leptin concentrations in raccoons. Clear seasonal variations were observed by ELISA, but not by RIA, with high values in autumn (3.46 ± 0.45 ng/ml) and low values in spring and summer (0.71 ± 0.07 ng/ml). Serum leptin concentrations in raccoons correlated positively with their body weight (r = 0.753) and body mass index (r = 0.755), corroborating our previous findings of a strong positive correlation between serum leptin concentrations and body fat content in dogs. Thus, the canine leptin ELISA is useful for assays of dog and raccoon leptin, and blood leptin is a good marker of nutritional condition in the species of Carnivora assayed in this study.
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UR - http://www.scopus.com/inward/citedby.url?scp=23044477383&partnerID=8YFLogxK
U2 - 10.1002/jez.a.185
DO - 10.1002/jez.a.185
M3 - Article
C2 - 15945076
AN - SCOPUS:23044477383
VL - 303
SP - 527
EP - 533
JO - Journal of Experimental Zoology Part A: Comparative Experimental Biology
JF - Journal of Experimental Zoology Part A: Comparative Experimental Biology
SN - 0022-104X
IS - 7
ER -