Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA

Haruki Shibata, Rie Akahane, Tsutomu Honjoh, Makoto Asano, Katsumi Mominoki, Kei Fujii, Masatsugu Suzuki, Noriyuki Ohtaishi, Katsumi Ishioka, Mohamed Ahmed, Mohamed Soliman, Kazuhiro Kimura, Masayuki Saito

Research output: Contribution to journalArticle

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Abstract

Several reports have been published on blood leptin concentrations in feral animals, including members of the Carnivora, using a commercially available multi-species radioimmunoassay (RIA) kit with anti-human leptin antibody. However, we observed weak immunoreactivity between recombinant canine leptin and anti-human leptin antibody, suggesting a limitation in the applicability of the RIA kit for leptin assays in Carnivora species. We tested the applicability of RIA and sandwich enzyme-linked immunosorbent assay (ELISA) with anti-canine leptin antibody to assay blood leptin in the dog (Canis familiaris) and the raccoon (Procyon lotor). When RIA was used for recombinant canine leptin and dog sera, values were much lower than those determined by ELISA at higher concentrations (> 10 ng/ml), while rather higher at lower concentrations (<2 ng/ml). A similar discrepancy between the two methods was found for serum leptin concentrations in raccoons. Clear seasonal variations were observed by ELISA, but not by RIA, with high values in autumn (3.46 ± 0.45 ng/ml) and low values in spring and summer (0.71 ± 0.07 ng/ml). Serum leptin concentrations in raccoons correlated positively with their body weight (r = 0.753) and body mass index (r = 0.755), corroborating our previous findings of a strong positive correlation between serum leptin concentrations and body fat content in dogs. Thus, the canine leptin ELISA is useful for assays of dog and raccoon leptin, and blood leptin is a good marker of nutritional condition in the species of Carnivora assayed in this study.

Original languageEnglish
Pages (from-to)527-533
Number of pages7
JournalJournal of Experimental Zoology Part A: Comparative Experimental Biology
Volume303
Issue number7
DOIs
Publication statusPublished - Jul 1 2005
Externally publishedYes

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Procyon lotor
blood serum
leptin
enzyme-linked immunosorbent assay
dogs
radioimmunoassays
Carnivora
antibodies
blood
assays
feral animals
sandwiches
body fat
body mass index

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA. / Shibata, Haruki; Akahane, Rie; Honjoh, Tsutomu; Asano, Makoto; Mominoki, Katsumi; Fujii, Kei; Suzuki, Masatsugu; Ohtaishi, Noriyuki; Ishioka, Katsumi; Ahmed, Mohamed; Soliman, Mohamed; Kimura, Kazuhiro; Saito, Masayuki.

In: Journal of Experimental Zoology Part A: Comparative Experimental Biology, Vol. 303, No. 7, 01.07.2005, p. 527-533.

Research output: Contribution to journalArticle

Shibata, H, Akahane, R, Honjoh, T, Asano, M, Mominoki, K, Fujii, K, Suzuki, M, Ohtaishi, N, Ishioka, K, Ahmed, M, Soliman, M, Kimura, K & Saito, M 2005, 'Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA', Journal of Experimental Zoology Part A: Comparative Experimental Biology, vol. 303, no. 7, pp. 527-533. https://doi.org/10.1002/jez.a.185
Shibata, Haruki ; Akahane, Rie ; Honjoh, Tsutomu ; Asano, Makoto ; Mominoki, Katsumi ; Fujii, Kei ; Suzuki, Masatsugu ; Ohtaishi, Noriyuki ; Ishioka, Katsumi ; Ahmed, Mohamed ; Soliman, Mohamed ; Kimura, Kazuhiro ; Saito, Masayuki. / Seasonal changes in serum leptin of the feral raccoon (Procyon lotor) determined by canine-leptin-specific ELISA. In: Journal of Experimental Zoology Part A: Comparative Experimental Biology. 2005 ; Vol. 303, No. 7. pp. 527-533.
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