Seasonal changes in luteal progesterone concentration and mRNA expressions of progesterone synthesis-related proteins in the Corpus Luteum of Mares

Keisuke Kozai, Takuo Hojo, Masashi Takahashi, Tomas J. Acosta, Yasuo Nambo, Kiyoshi Okuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although circulating progesterone (P4) levels tend to change with the season, little is known about the seasonal changes of P4 synthesis-related proteins in the corpus luteum (CL) of mares. To examine these changes, seventy-four ovaries containing a CL were collected from Anglo-Norman mares at a local abattoir in Kumamoto, Japan (~N32°), five times during one year. The stages of the CLs were classified as early, mid and regressed by macroscopic observation of the CL and follicles. The mid CL, which had the highest P4 concentration, was used to evaluate the seasonal changes in P4 synthesis. The luteal P4 concentration and mRNA expression of luteinizing hormone receptor (LHCGR) were lowest during early winter and highest during late winter. The mRNA expressions of steroidogenic acute regulatory protein (StAR), P450 cholesterol side-chain cleavage enzyme (P450scc) and 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD) were lowest during early winter and increased during late winter. These results suggest that P4 synthesis in the CL is affected by the seasonal changes in the mRNA expressions of P4 synthesis-related proteins in mares.

Original languageEnglish
Pages (from-to)393-397
Number of pages5
JournalJournal of Reproduction and Development
Volume58
Issue number4
DOIs
Publication statusPublished - 2012

Keywords

  • Corpus luteum
  • Mares
  • Progesterone
  • Reproductive seasonality
  • Steroidogenesis

ASJC Scopus subject areas

  • Animal Science and Zoology

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