Seasonal abscisic acid signal and a basic leucine zipper transcription factor, DkbZIP5, regulate proanthocyanidin biosynthesis in persimmon fruit

Takashi Akagi, Ayako Katayama-Ikegami, Shozo Kobayashi, Akihiko Sato, Atsushi Kono, Keizo Yonemori

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Proanthocyanidins (PAs) are secondary metabolites that contribute to plant protection and crop quality. Persimmon (Diospyros kaki) has a unique characteristic of accumulating large amounts of PAs, particularly in its fruit. Normal astringent-type and mutant nonastringent-type fruits show different PA accumulation patterns depending on the seasonal expression patterns of DkMyb4, which is a Myb transcription factor (TF) regulating many PA pathway genes in persimmon. In this study, attempts were made to identify the factors involved in DkMyb4 expression and the resultant PA accumulation in persimmon fruit. Treatment with abscisic acid (ABA) and an ABA biosynthesis inhibitor resulted in differential changes in the expression patterns of DkMyb4 and PA biosynthesis in astringent-type and nonastringent-type fruits depending on the development stage. To obtain an ABA-signaling TF, we isolated a full-length basic leucine zipper (bZIP) TF, DkbZIP5, which is highly expressed in persimmon fruit. We also showed that ectopic DkbZIP5 overexpression in persimmon calluses induced the up-regulation of DkMyb4 and the resultant PA biosynthesis. In addition, a detailed molecular characterization using the electrophoretic mobility shift assay and transient reporter assay indicated that DkbZIP5 recognized ABA-responsive elements in the promoter region of DkMyb4 and acted as a direct regulator of DkMyb4 in an ABA-dependent manner. These results suggest that ABA signals may be involved in PA biosynthesis in persimmon fruit via DkMyb4 activation by DkbZIP5.

Original languageEnglish
Pages (from-to)1089-1102
Number of pages14
JournalPlant physiology
Volume158
Issue number2
DOIs
Publication statusPublished - Feb 1 2012
Externally publishedYes

Fingerprint

Diospyros
Basic-Leucine Zipper Transcription Factors
Proanthocyanidins
Abscisic Acid
persimmons
proanthocyanidins
abscisic acid
Fruit
biosynthesis
Astringents
fruits
Transcription Factors
transcription factors
Diospyros kaki
crop quality
proanthocyanidin
basic-leucine zipper transcription factors
Bony Callus
Electrophoretic Mobility Shift Assay
assays

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Seasonal abscisic acid signal and a basic leucine zipper transcription factor, DkbZIP5, regulate proanthocyanidin biosynthesis in persimmon fruit. / Akagi, Takashi; Katayama-Ikegami, Ayako; Kobayashi, Shozo; Sato, Akihiko; Kono, Atsushi; Yonemori, Keizo.

In: Plant physiology, Vol. 158, No. 2, 01.02.2012, p. 1089-1102.

Research output: Contribution to journalArticle

Akagi, Takashi ; Katayama-Ikegami, Ayako ; Kobayashi, Shozo ; Sato, Akihiko ; Kono, Atsushi ; Yonemori, Keizo. / Seasonal abscisic acid signal and a basic leucine zipper transcription factor, DkbZIP5, regulate proanthocyanidin biosynthesis in persimmon fruit. In: Plant physiology. 2012 ; Vol. 158, No. 2. pp. 1089-1102.
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