Screening of edible Japanese plants for suppressive effects on phorbol ester-induced superoxide generation in differentiated HL-60 cells and AS52 cells

Ha Won Kim, Akira Murakami, Yoshimasa Nakamura, Hajime Ohigashi

Research output: Contribution to journalArticle

30 Citations (Scopus)


Epithelial xanthine oxidase (XOD) is one of the major enzymes responsible for superoxide (O2-) generation, which is involved in oxidative stress. However, there are few known reports of a convenient bioassay to detect cellular XOD activity. We tested several cell lines, and found that AS52, from Chinese hamster ovary cells, produced a significant level of O2- in response to 12-O-tetradecanoylphorbol 13-acetate (TPA), and this activity was markedly inhibited by allopurinol, an XOD inhibitor. Using AS52 cells and differentiated HL-60 cells, we conducted screening tests of edible Japanese plant extracts for their inhibitory activities toward TPA-induced O2- generation from both reduced nicotinamide adenine dinucleotide oxidase (HL-60) and XOD (AS52). Notably, the extracts from mioga ginger, rape, avocado, carrot, turnip, taro, and shimeji showed potent inhibition of O2- generation in both cell lines. These results suggest that several edible Japanese plants carry a significant antioxidative and cancer preventive potential.

Original languageEnglish
Pages (from-to)7-16
Number of pages10
JournalCancer Letters
Issue number1
Publication statusPublished - Feb 8 2002
Externally publishedYes



  • AS52 cells
  • Cancer chemoprevention
  • HL-60 cells
  • Reduced nicotinamide adenine dinucleotide phosphate oxidase
  • Superoxide
  • Xanthine oxidase

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this