Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264.7 cells

Oe Kyung Kim, Akira Murakami, Yoshimasa Nakamura, Hajime Ohigashi

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

A total of 48 (60 test samples) species of plants commonly eaten in Japan were randomly collected and their methanol extracts were tested for in vitro nitric oxide (NO) generation inhibitory activities in a murine macrophage cell line, RAW 264.7, stimulated with both lipopulysaccharide (LPS, 100 ng/ml) and interferon-y (IFN-y, 100 U/ml). Seventeen (28.3%) of the 48 extracts strongly inhibited NO generation at a concentration of 200 μg/ml by 70% or more with significant cell viability (>50%). The extracts from avocado, tare, red turnip, sereves, komatsuna, basil, mitsuba and Chinese mustard markedly inhibited iNOS activity. These results suggest that a wide variety of edible plants in Japan contain the secondary metabolites carrying cancer preventive activity through reduction of excess amounts of NO.

Original languageEnglish
Pages (from-to)199-207
Number of pages9
JournalCancer Letters
Volume125
Issue number1-2
DOIs
Publication statusPublished - Mar 13 1998
Externally publishedYes

Keywords

  • Cancer chemoprevention
  • Edible plants
  • Nitric oxide
  • Raw 264.7 cells

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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