Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264.7 cells

Oe Kyung Kim; Akira Murakami; Yoshimasa Nakamura; Hajime Ohigashi

doi:10.1016/S0304-3835(97)00513-2

Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264.7 cells

Oe Kyung Kim, Akira Murakami, Yoshimasa Nakamura, Hajime Ohigashi

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

A total of 48 (60 test samples) species of plants commonly eaten in Japan were randomly collected and their methanol extracts were tested for in vitro nitric oxide (NO) generation inhibitory activities in a murine macrophage cell line, RAW 264.7, stimulated with both lipopulysaccharide (LPS, 100 ng/ml) and interferon-y (IFN-y, 100 U/ml). Seventeen (28.3%) of the 48 extracts strongly inhibited NO generation at a concentration of 200 μg/ml by 70% or more with significant cell viability (>50%). The extracts from avocado, tare, red turnip, sereves, komatsuna, basil, mitsuba and Chinese mustard markedly inhibited iNOS activity. These results suggest that a wide variety of edible plants in Japan contain the secondary metabolites carrying cancer preventive activity through reduction of excess amounts of NO.

Original language	English
Pages (from-to)	199-207
Number of pages	9
Journal	Cancer Letters
Volume	125
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(97)00513-2
Publication status	Published - Mar 13 1998
Externally published	Yes

Keywords

Cancer chemoprevention
Edible plants
Nitric oxide
Raw 264.7 cells

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0304-3835(97)00513-2

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title = "Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264.7 cells",

abstract = "A total of 48 (60 test samples) species of plants commonly eaten in Japan were randomly collected and their methanol extracts were tested for in vitro nitric oxide (NO) generation inhibitory activities in a murine macrophage cell line, RAW 264.7, stimulated with both lipopulysaccharide (LPS, 100 ng/ml) and interferon-y (IFN-y, 100 U/ml). Seventeen (28.3%) of the 48 extracts strongly inhibited NO generation at a concentration of 200 μg/ml by 70% or more with significant cell viability (>50%). The extracts from avocado, tare, red turnip, sereves, komatsuna, basil, mitsuba and Chinese mustard markedly inhibited iNOS activity. These results suggest that a wide variety of edible plants in Japan contain the secondary metabolites carrying cancer preventive activity through reduction of excess amounts of NO.",

keywords = "Cancer chemoprevention, Edible plants, Nitric oxide, Raw 264.7 cells",

author = "Kim, {Oe Kyung} and Akira Murakami and Yoshimasa Nakamura and Hajime Ohigashi",

note = "Funding Information: This study was partly supported by Grants-in-Aid from the Ministry of Health and Welfare for the Second Term Comprehensive 10-Year Strategy for Cancer Control, Japan (H.O.) and from the Ministry of Education, Science, and Culture of Japan (A.M.) and by subsidies from Takeda Food Products (H.O.). O.-K.K. thanks the Saneyoshi Scholarship Foundation for financial support. We also thank Dr K. Tatsumi of Ohtsuka Pharmaceutical Company for his kind gift of RAW 264.7 cells and valuable comments on the bioassay.",

year = "1998",

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doi = "10.1016/S0304-3835(97)00513-2",

language = "English",

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pages = "199--207",

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AU - Kim, Oe Kyung

AU - Murakami, Akira

AU - Nakamura, Yoshimasa

AU - Ohigashi, Hajime

N1 - Funding Information: This study was partly supported by Grants-in-Aid from the Ministry of Health and Welfare for the Second Term Comprehensive 10-Year Strategy for Cancer Control, Japan (H.O.) and from the Ministry of Education, Science, and Culture of Japan (A.M.) and by subsidies from Takeda Food Products (H.O.). O.-K.K. thanks the Saneyoshi Scholarship Foundation for financial support. We also thank Dr K. Tatsumi of Ohtsuka Pharmaceutical Company for his kind gift of RAW 264.7 cells and valuable comments on the bioassay.

PY - 1998/3/13

Y1 - 1998/3/13

N2 - A total of 48 (60 test samples) species of plants commonly eaten in Japan were randomly collected and their methanol extracts were tested for in vitro nitric oxide (NO) generation inhibitory activities in a murine macrophage cell line, RAW 264.7, stimulated with both lipopulysaccharide (LPS, 100 ng/ml) and interferon-y (IFN-y, 100 U/ml). Seventeen (28.3%) of the 48 extracts strongly inhibited NO generation at a concentration of 200 μg/ml by 70% or more with significant cell viability (>50%). The extracts from avocado, tare, red turnip, sereves, komatsuna, basil, mitsuba and Chinese mustard markedly inhibited iNOS activity. These results suggest that a wide variety of edible plants in Japan contain the secondary metabolites carrying cancer preventive activity through reduction of excess amounts of NO.

AB - A total of 48 (60 test samples) species of plants commonly eaten in Japan were randomly collected and their methanol extracts were tested for in vitro nitric oxide (NO) generation inhibitory activities in a murine macrophage cell line, RAW 264.7, stimulated with both lipopulysaccharide (LPS, 100 ng/ml) and interferon-y (IFN-y, 100 U/ml). Seventeen (28.3%) of the 48 extracts strongly inhibited NO generation at a concentration of 200 μg/ml by 70% or more with significant cell viability (>50%). The extracts from avocado, tare, red turnip, sereves, komatsuna, basil, mitsuba and Chinese mustard markedly inhibited iNOS activity. These results suggest that a wide variety of edible plants in Japan contain the secondary metabolites carrying cancer preventive activity through reduction of excess amounts of NO.

KW - Cancer chemoprevention

KW - Edible plants

KW - Nitric oxide

KW - Raw 264.7 cells

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Screening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 264.7 cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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