Screening of bacterial DNA in bile sampled from healthy dogs and dogs suffering from liver-or gallbladder-associated disease

Sakurako Neo, Iyo Takemura-Uchiyama, Jumpei Uchiyama, Hironobu Murakami, Ayaka Shima, Hideki Kayanuma, Taiki Yokoyama, Satoshi Takagi, Eiichi Kanai, Masaharu Hisasue

Research output: Contribution to journalComment/debatepeer-review

Abstract

Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver-or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCR-positive samples showed that the microbial diversity differed between liver-and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs.

Original languageEnglish
Pages (from-to)1019-1022
Number of pages4
JournalJournal of Veterinary Medical Science
Volume84
Issue number7
DOIs
Publication statusPublished - 2022
Externally publishedYes

Keywords

  • bile
  • bile microbiota
  • gallbladder
  • hospitalized dog
  • laboratory dog

ASJC Scopus subject areas

  • veterinary(all)

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