Screening for genes up-regulated in 5/6 nephrectomized mouse kidney

Hong Zhang, Jun Wada, Yashpal S. Kanwar, Yoshinori Tsuchiyama, Keita Hiragushi, Kazuyuki Hida, Kenichi Shikata, Hirofumi Makino

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background. In diabetic and nondiabetic renal diseases, glomerular hyperfiltration is believed to play a central role in the subsequent progression of glomerulosclerosis and interstitial renal scarring. To identify genes involved in the process of hyperfiltration and hypertrophy, a polymerase chain reaction (PCR)-based subtraction method, that is, representational difference analysis of cDNA (cDNA-RDA), was employed. Methods. Ten-week-old ICR mice were 5/6 nephrectomized and sham operated. After two weeks, mRNAs were isolated from control and remnant kidneys and were subjected to the cDNA-RDA procedure. Results. We identified 10 known and 9 novel genes. Among 19 clones, 12 clones (8 known and 4 novel) showed 1.5- to 6-fold up-regulation by Northern blot analyses. The remaining seven clones were rarely expressed genes and were barely detected by Northern blot analyses, and their up-regulated expression was confirmed by Southern blot analysis using the PCR-amplified representative amplicons. The known genes included kidney androgen-regulated protein, major urinary protein, lysozyme M, metalloproteinase-3 tissue inhibitor, chaperonin 10, cytochrome oxidase I, ε-sarcoglycan, ribosomal protein S3a, G-proteinγ10 subunit, and splicing factor 9G8. All of the isolated known genes have not been reported to be up- regulated in the nephrectomized mouse kidney and suggest the possible role of androgen action, mitochondrial functions, matrix metabolism, cell-matrix interactions, and intracellular signaling events in the initiation of the progressive renal injury of the remnant kidney. Furthermore, cDNA-RDA facilitates the discovery of novel genes, including two kidney-specific genes. Conclusions. The isolated known and novel genes may be involved in the pathobiological process of initial hyperfiltration and hypertrophy of remnant kidney.

Original languageEnglish
Pages (from-to)549-558
Number of pages10
JournalKidney International
Volume56
Issue number2
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • Gene expression
  • Glomerular hyperfiltration
  • Nephrectomy
  • Remnant kidney
  • Subtractive screening

ASJC Scopus subject areas

  • Nephrology

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