TY - JOUR
T1 - Screening for genes up-regulated in 5/6 nephrectomized mouse kidney
AU - Zhang, Hong
AU - Wada, Jun
AU - Kanwar, Yashpal S.
AU - Tsuchiyama, Yoshinori
AU - Hiragushi, Keita
AU - Hida, Kazuyuki
AU - Shikata, Kenichi
AU - Makino, Hirofumi
N1 - Funding Information:
This work was supported by the Uehara Memorial Foundation, The Naito Foundation, ONO Medical Foundation, Grant-in-Aid for Encouragement of Young Scientists, Ministry of Education, Science and Culture, Japan (10770199) to Jun Wada and Grant-in-Aid for Scientific Research (B), Ministry of Education, Science and Culture, Japan (11470218) and Uehara Memorial Foundation to H. Makino. H. Zhang was supported by an International Society of Nephrology/Kirin Fellowship Award.
PY - 1999
Y1 - 1999
N2 - Background. In diabetic and nondiabetic renal diseases, glomerular hyperfiltration is believed to play a central role in the subsequent progression of glomerulosclerosis and interstitial renal scarring. To identify genes involved in the process of hyperfiltration and hypertrophy, a polymerase chain reaction (PCR)-based subtraction method, that is, representational difference analysis of cDNA (cDNA-RDA), was employed. Methods. Ten-week-old ICR mice were 5/6 nephrectomized and sham operated. After two weeks, mRNAs were isolated from control and remnant kidneys and were subjected to the cDNA-RDA procedure. Results. We identified 10 known and 9 novel genes. Among 19 clones, 12 clones (8 known and 4 novel) showed 1.5- to 6-fold up-regulation by Northern blot analyses. The remaining seven clones were rarely expressed genes and were barely detected by Northern blot analyses, and their up-regulated expression was confirmed by Southern blot analysis using the PCR-amplified representative amplicons. The known genes included kidney androgen-regulated protein, major urinary protein, lysozyme M, metalloproteinase-3 tissue inhibitor, chaperonin 10, cytochrome oxidase I, ε-sarcoglycan, ribosomal protein S3a, G-proteinγ10 subunit, and splicing factor 9G8. All of the isolated known genes have not been reported to be up- regulated in the nephrectomized mouse kidney and suggest the possible role of androgen action, mitochondrial functions, matrix metabolism, cell-matrix interactions, and intracellular signaling events in the initiation of the progressive renal injury of the remnant kidney. Furthermore, cDNA-RDA facilitates the discovery of novel genes, including two kidney-specific genes. Conclusions. The isolated known and novel genes may be involved in the pathobiological process of initial hyperfiltration and hypertrophy of remnant kidney.
AB - Background. In diabetic and nondiabetic renal diseases, glomerular hyperfiltration is believed to play a central role in the subsequent progression of glomerulosclerosis and interstitial renal scarring. To identify genes involved in the process of hyperfiltration and hypertrophy, a polymerase chain reaction (PCR)-based subtraction method, that is, representational difference analysis of cDNA (cDNA-RDA), was employed. Methods. Ten-week-old ICR mice were 5/6 nephrectomized and sham operated. After two weeks, mRNAs were isolated from control and remnant kidneys and were subjected to the cDNA-RDA procedure. Results. We identified 10 known and 9 novel genes. Among 19 clones, 12 clones (8 known and 4 novel) showed 1.5- to 6-fold up-regulation by Northern blot analyses. The remaining seven clones were rarely expressed genes and were barely detected by Northern blot analyses, and their up-regulated expression was confirmed by Southern blot analysis using the PCR-amplified representative amplicons. The known genes included kidney androgen-regulated protein, major urinary protein, lysozyme M, metalloproteinase-3 tissue inhibitor, chaperonin 10, cytochrome oxidase I, ε-sarcoglycan, ribosomal protein S3a, G-proteinγ10 subunit, and splicing factor 9G8. All of the isolated known genes have not been reported to be up- regulated in the nephrectomized mouse kidney and suggest the possible role of androgen action, mitochondrial functions, matrix metabolism, cell-matrix interactions, and intracellular signaling events in the initiation of the progressive renal injury of the remnant kidney. Furthermore, cDNA-RDA facilitates the discovery of novel genes, including two kidney-specific genes. Conclusions. The isolated known and novel genes may be involved in the pathobiological process of initial hyperfiltration and hypertrophy of remnant kidney.
KW - Gene expression
KW - Glomerular hyperfiltration
KW - Nephrectomy
KW - Remnant kidney
KW - Subtractive screening
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U2 - 10.1046/j.1523-1755.1999.00561.x
DO - 10.1046/j.1523-1755.1999.00561.x
M3 - Article
C2 - 10432394
AN - SCOPUS:0032782374
VL - 56
SP - 549
EP - 558
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 2
ER -