Scavenger receptor type BI potentiates reverse cholesterol transport system by removing cholesterol ester from HDL

Makoto Kinoshita, Mineko Fujita, Shinichi Usui, Yoko Maeda, Mikiko Kudo, Daisuke Hirota, Takaoki Suda, Masanari Taki, Mitsuyo Okazaki, Tamio Teramoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

High-density lipoprotein (HDL) plays an important role in reverse cholesterol transport by removing accumulated cholesterol from extrahepatic tissues. Subsequently, cholesterol ester (CE) on HDL in humans is transported to apolipoprotein B-containing lipoproteins by cholesteryl ester transfer protein (CETP). CETP deficiency, which is common in the Japanese population, leads to a marked increase in HDL-cholesterol levels due to impaired CE transport from HDL to LDL. It has been reported that the HDL observed in CETP deficiency is an atherogenic lipoprotein, as it contains a large amount of CE. Scavenger receptor class B type I (SR-BI) has been found to be an authentic HDL receptor that mediates the selective uptake of HDL CE and the bi-directional transfer of free cholesterol between HDL and cells. In the present study, the interaction between SR-BI and CE-rich HDL from CETP-deficient patient was studied in order to evaluate the anti-atherosclerotic role of SR-BI in relation to CE uptake and reverse cholesterol transport. When CE-rich HDL was added to the medium of SR-BI-transfected CHO (SR-BI CHO) cells, more CE accumulated in SR-BI CHO cells compared to control HDL. In contrast, the amount of cholesterol efflux from SR-BI CHO cells into HDL was almost the same between the two HDLs. Therefore, when CE-rich HDL was added to the medium of SR-BI CHO cells, the intracellular CE content increased significantly. Moreover, the particle size of HDL in CETP-deficient patient decreased significantly when the HDL was added to the medium of SR-BI CHO cells, and this HDL showed an increment of CE efflux from foam cells. These results indicate that SR-BI reduces the cholesterol content and size of the CE-rich HDL from CETP deficiency, which ultimately activate reverse cholesterol transport system.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalAtherosclerosis
Volume173
Issue number2
DOIs
Publication statusPublished - Apr 2004

Fingerprint

Scavenger Receptors
Cholesterol Esters
HDL Lipoproteins
CD36 Antigens
Cholesterol
CHO Cells
Cholesterol Ester Transfer Proteins
HDL Cholesterol
Lipoproteins
Foam Cells
Apolipoproteins B
Particle Size

Keywords

  • Apo
  • Apolipoprotein
  • Atherosclerosis
  • CE
  • CETP
  • CETP deficiency
  • Cholesterol ester
  • Cholesteryl ester transfer protein
  • LCAT
  • Lecithin:cholesterol acyltransferase
  • Lipoprotein-deficient serum
  • LPDS
  • Scavenger receptor class B type I
  • SR-BI

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Scavenger receptor type BI potentiates reverse cholesterol transport system by removing cholesterol ester from HDL. / Kinoshita, Makoto; Fujita, Mineko; Usui, Shinichi; Maeda, Yoko; Kudo, Mikiko; Hirota, Daisuke; Suda, Takaoki; Taki, Masanari; Okazaki, Mitsuyo; Teramoto, Tamio.

In: Atherosclerosis, Vol. 173, No. 2, 04.2004, p. 197-202.

Research output: Contribution to journalArticle

Kinoshita, M, Fujita, M, Usui, S, Maeda, Y, Kudo, M, Hirota, D, Suda, T, Taki, M, Okazaki, M & Teramoto, T 2004, 'Scavenger receptor type BI potentiates reverse cholesterol transport system by removing cholesterol ester from HDL', Atherosclerosis, vol. 173, no. 2, pp. 197-202. https://doi.org/10.1016/j.atherosclerosis.2003.12.014
Kinoshita, Makoto ; Fujita, Mineko ; Usui, Shinichi ; Maeda, Yoko ; Kudo, Mikiko ; Hirota, Daisuke ; Suda, Takaoki ; Taki, Masanari ; Okazaki, Mitsuyo ; Teramoto, Tamio. / Scavenger receptor type BI potentiates reverse cholesterol transport system by removing cholesterol ester from HDL. In: Atherosclerosis. 2004 ; Vol. 173, No. 2. pp. 197-202.
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abstract = "High-density lipoprotein (HDL) plays an important role in reverse cholesterol transport by removing accumulated cholesterol from extrahepatic tissues. Subsequently, cholesterol ester (CE) on HDL in humans is transported to apolipoprotein B-containing lipoproteins by cholesteryl ester transfer protein (CETP). CETP deficiency, which is common in the Japanese population, leads to a marked increase in HDL-cholesterol levels due to impaired CE transport from HDL to LDL. It has been reported that the HDL observed in CETP deficiency is an atherogenic lipoprotein, as it contains a large amount of CE. Scavenger receptor class B type I (SR-BI) has been found to be an authentic HDL receptor that mediates the selective uptake of HDL CE and the bi-directional transfer of free cholesterol between HDL and cells. In the present study, the interaction between SR-BI and CE-rich HDL from CETP-deficient patient was studied in order to evaluate the anti-atherosclerotic role of SR-BI in relation to CE uptake and reverse cholesterol transport. When CE-rich HDL was added to the medium of SR-BI-transfected CHO (SR-BI CHO) cells, more CE accumulated in SR-BI CHO cells compared to control HDL. In contrast, the amount of cholesterol efflux from SR-BI CHO cells into HDL was almost the same between the two HDLs. Therefore, when CE-rich HDL was added to the medium of SR-BI CHO cells, the intracellular CE content increased significantly. Moreover, the particle size of HDL in CETP-deficient patient decreased significantly when the HDL was added to the medium of SR-BI CHO cells, and this HDL showed an increment of CE efflux from foam cells. These results indicate that SR-BI reduces the cholesterol content and size of the CE-rich HDL from CETP deficiency, which ultimately activate reverse cholesterol transport system.",
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AU - Hirota, Daisuke

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AB - High-density lipoprotein (HDL) plays an important role in reverse cholesterol transport by removing accumulated cholesterol from extrahepatic tissues. Subsequently, cholesterol ester (CE) on HDL in humans is transported to apolipoprotein B-containing lipoproteins by cholesteryl ester transfer protein (CETP). CETP deficiency, which is common in the Japanese population, leads to a marked increase in HDL-cholesterol levels due to impaired CE transport from HDL to LDL. It has been reported that the HDL observed in CETP deficiency is an atherogenic lipoprotein, as it contains a large amount of CE. Scavenger receptor class B type I (SR-BI) has been found to be an authentic HDL receptor that mediates the selective uptake of HDL CE and the bi-directional transfer of free cholesterol between HDL and cells. In the present study, the interaction between SR-BI and CE-rich HDL from CETP-deficient patient was studied in order to evaluate the anti-atherosclerotic role of SR-BI in relation to CE uptake and reverse cholesterol transport. When CE-rich HDL was added to the medium of SR-BI-transfected CHO (SR-BI CHO) cells, more CE accumulated in SR-BI CHO cells compared to control HDL. In contrast, the amount of cholesterol efflux from SR-BI CHO cells into HDL was almost the same between the two HDLs. Therefore, when CE-rich HDL was added to the medium of SR-BI CHO cells, the intracellular CE content increased significantly. Moreover, the particle size of HDL in CETP-deficient patient decreased significantly when the HDL was added to the medium of SR-BI CHO cells, and this HDL showed an increment of CE efflux from foam cells. These results indicate that SR-BI reduces the cholesterol content and size of the CE-rich HDL from CETP deficiency, which ultimately activate reverse cholesterol transport system.

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