Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi

Dai Tsuchiya, Hironori Koga, Masatoki Taga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new method for scanning electron microscopy (SEM) of fungal mitotic nuclei and chromosomes was established for two ascomycetes, Cochliobolus heterostrophus and Neurospora crassa. Nuclei and chromosomes discharged from germling cells by the germ-tube burst method were spread on a surface of a glass slide. The spreads were impregnated with osmium-thiocarbohydrazide for conductive staining, followed by coating with platinum, and observed by field-emission SEM, Ultrastructure of fungal chromosomes and nuclei was visualized by SEM for the first time.

Original languageEnglish
Pages (from-to)208-210
Number of pages3
JournalMycologia
Volume96
Issue number2
Publication statusPublished - Mar 2004

Fingerprint

Electron Scanning Microscopy
chromosome
Ascomycota
scanning electron microscopy
Fungi
Chromosomes
fungus
chromosomes
Fungal Chromosomes
fungi
osmium
Neurospora crassa
platinum
germ tube
ultrastructure
Platinum
Germ Cells
coatings
Glass
glass

Keywords

  • Cochliobolus heterostrophus
  • Germ-tube burst method
  • Mitosis
  • Neurospora crassa

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Plant Science

Cite this

Tsuchiya, D., Koga, H., & Taga, M. (2004). Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi. Mycologia, 96(2), 208-210.

Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi. / Tsuchiya, Dai; Koga, Hironori; Taga, Masatoki.

In: Mycologia, Vol. 96, No. 2, 03.2004, p. 208-210.

Research output: Contribution to journalArticle

Tsuchiya, D, Koga, H & Taga, M 2004, 'Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi', Mycologia, vol. 96, no. 2, pp. 208-210.
Tsuchiya, Dai ; Koga, Hironori ; Taga, Masatoki. / Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi. In: Mycologia. 2004 ; Vol. 96, No. 2. pp. 208-210.
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