Scanning electron microscopy of mitotic nuclei and chromosomes in filamentous fungi

Dai Tsuchiya, Hironori Koga, Masatoki Taga

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new method for scanning electron microscopy (SEM) of fungal mitotic nuclei and chromosomes was established for two ascomycetes, Cochliobolus heterostrophus and Neurospora crassa. Nuclei and chromosomes discharged from germling cells by the germ-tube burst method were spread on a surface of a glass slide. The spreads were impregnated with osmium-thiocarbohydrazide for conductive staining, followed by coating with platinum, and observed by field-emission SEM, Ultrastructure of fungal chromosomes and nuclei was visualized by SEM for the first time.

Original languageEnglish
Pages (from-to)208-210
Number of pages3
JournalMycologia
Volume96
Issue number2
DOIs
Publication statusPublished - Jan 1 2004

Keywords

  • Cochliobolus heterostrophus
  • Germ-tube burst method
  • Mitosis
  • Neurospora crassa

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Molecular Biology
  • Genetics
  • Cell Biology

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