Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

Koyo Watanabe, Koji Matsuura, Fukukazu Kawata, Kotaro Nagata, Jun Ning, Hiroshi Kano

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.

Original languageEnglish
Pages (from-to)354-359
Number of pages6
JournalBiomedical Optics Express
Volume3
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

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Cell Adhesion
Microscopy
adhesion
microscopy
Focal Adhesions
scanning
cells
microscopes
Muscle Cells
muscle cells
marking
mice
high resolution

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

Cite this

Watanabe, K., Matsuura, K., Kawata, F., Nagata, K., Ning, J., & Kano, H. (2012). Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites. Biomedical Optics Express, 3(2), 354-359. https://doi.org/10.1364/BOE.3.000354

Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites. / Watanabe, Koyo; Matsuura, Koji; Kawata, Fukukazu; Nagata, Kotaro; Ning, Jun; Kano, Hiroshi.

In: Biomedical Optics Express, Vol. 3, No. 2, 01.02.2012, p. 354-359.

Research output: Contribution to journalArticle

Watanabe, K, Matsuura, K, Kawata, F, Nagata, K, Ning, J & Kano, H 2012, 'Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites', Biomedical Optics Express, vol. 3, no. 2, pp. 354-359. https://doi.org/10.1364/BOE.3.000354
Watanabe, Koyo ; Matsuura, Koji ; Kawata, Fukukazu ; Nagata, Kotaro ; Ning, Jun ; Kano, Hiroshi. / Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites. In: Biomedical Optics Express. 2012 ; Vol. 3, No. 2. pp. 354-359.
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