Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

Koyo Watanabe; Koji Matsuura; Fukukazu Kawata; Kotaro Nagata; Jun Ning; Hiroshi Kano

doi:10.1364/BOE.3.000354

Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

Koyo Watanabe, Koji Matsuura, Fukukazu Kawata, Kotaro Nagata, Jun Ning, Hiroshi Kano

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.

Original language	English
Pages (from-to)	354-359
Number of pages	6
Journal	Biomedical Optics Express
Volume	3
Issue number	2
DOIs	https://doi.org/10.1364/BOE.3.000354
Publication status	Published - Feb 1 2012
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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AU - Matsuura, Koji

AU - Kawata, Fukukazu

AU - Nagata, Kotaro

AU - Ning, Jun

AU - Kano, Hiroshi

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AB - We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.

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Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

Abstract

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