SAXS and SANS observations of abnormal aggregation of human •-crystallin

Masaaki Sugiyama, Norihiko Fujii, Yukio Morimoto, Keiji Itoh, Kazuhiro Mori, Toshiharu Fukunaga, Noriko Fujii

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4 Citations (Scopus)

Abstract

Aggregation states of human α-crystallins are observed complementarily using small-angle X-ray and small-angle neutron scatterings (SAXS and SANS). Infant α-crystallin is almost a monodispersed system of the aggregates with gyration radius of ca. 60 Å, which is a normal aggregate. On the other hand, the aged and cataract α-crystallins have not only the normal but also the larger aggregates. In the aged alpha;-crystallin, the normal aggregate is a major component, but in the cataract α-crystallin the larger ones are dominant. Both αA- and alpha;B-crystallins, which are subunits of α-crystallin, also form an aggregate with the size close to the normal aggregate. Under UV irradiation, only aggregates of αB-crystallin undergo further aggregation. Therefore, considering increase of ratio of αB-crystallin in the aggregate of alpha;-crystallin as aging, the abnormal aggregation (formation of the huge aggregates) mainly results in the further aggregation of alpha;B-crystallin caused by external stresses.

Original languageEnglish
Pages (from-to)1380-1388
Number of pages9
JournalChemistry and Biodiversity
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 2010

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Molecular Medicine
  • Molecular Biology

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    Sugiyama, M., Fujii, N., Morimoto, Y., Itoh, K., Mori, K., Fukunaga, T., & Fujii, N. (2010). SAXS and SANS observations of abnormal aggregation of human •-crystallin. Chemistry and Biodiversity, 7(6), 1380-1388. https://doi.org/10.1002/cbdv.200900332