Abstract
Nax is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Nax has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Nax by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Nax in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Nax or depletion of SAP97 resulted in a decrease in cell-surface Nax, which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Nax channels at the plasma membrane. Structured summary of protein interactions: Nax physically interacts with SAP97 by anti tag coimmunoprecipitation (View interaction) CNRasGEF binds to Nax by protein array (View interaction) Nax and SAP97 colocalize by fluorescence microscopy (View interaction) GIPC1 binds to Nax by protein array (View interaction) ZO-1 binds to Nax by protein array (View interaction) SAP97 binds to Nax by protein array (View interaction) Densin-180 binds to Nax by protein array (View interaction) Beta-1-syntrophin binds to Nax by protein array (View interaction) ERBIN binds to Nax by protein array (View interaction) Nax physically interacts with SAP97 by pull down (View interaction) Lnx1 binds to Nax by protein array (View interaction) nNOS binds to Nax by protein array (View interaction)
| Original language | English |
|---|---|
| Pages (from-to) | 3805-3812 |
| Number of pages | 8 |
| Journal | FEBS Letters |
| Volume | 586 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - Nov 2 2012 |
| Externally published | Yes |
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Keywords
- Astrocyte
- MAGUK protein
- PDZ array
- PDZ-binding motif
- Sodium channel
- Subfornical organ
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
Cite this
SAP97 promotes the stability of Nax channels at the plasma membrane. / Matsumoto, Masahito; Fujikawa, Akihiro; Suzuki, Ryoko; Shimizu, Hidetada; Kuboyama, Kazuya; Hiyama, Takeshi Y.; Hall, Randy A.; Noda, Masaharu.
In: FEBS Letters, Vol. 586, No. 21, 02.11.2012, p. 3805-3812.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SAP97 promotes the stability of Nax channels at the plasma membrane
AU - Matsumoto, Masahito
AU - Fujikawa, Akihiro
AU - Suzuki, Ryoko
AU - Shimizu, Hidetada
AU - Kuboyama, Kazuya
AU - Hiyama, Takeshi Y.
AU - Hall, Randy A.
AU - Noda, Masaharu
PY - 2012/11/2
Y1 - 2012/11/2
N2 - Nax is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Nax has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Nax by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Nax in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Nax or depletion of SAP97 resulted in a decrease in cell-surface Nax, which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Nax channels at the plasma membrane. Structured summary of protein interactions: Nax physically interacts with SAP97 by anti tag coimmunoprecipitation (View interaction) CNRasGEF binds to Nax by protein array (View interaction) Nax and SAP97 colocalize by fluorescence microscopy (View interaction) GIPC1 binds to Nax by protein array (View interaction) ZO-1 binds to Nax by protein array (View interaction) SAP97 binds to Nax by protein array (View interaction) Densin-180 binds to Nax by protein array (View interaction) Beta-1-syntrophin binds to Nax by protein array (View interaction) ERBIN binds to Nax by protein array (View interaction) Nax physically interacts with SAP97 by pull down (View interaction) Lnx1 binds to Nax by protein array (View interaction) nNOS binds to Nax by protein array (View interaction)
AB - Nax is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Nax has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Nax by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Nax in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Nax or depletion of SAP97 resulted in a decrease in cell-surface Nax, which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Nax channels at the plasma membrane. Structured summary of protein interactions: Nax physically interacts with SAP97 by anti tag coimmunoprecipitation (View interaction) CNRasGEF binds to Nax by protein array (View interaction) Nax and SAP97 colocalize by fluorescence microscopy (View interaction) GIPC1 binds to Nax by protein array (View interaction) ZO-1 binds to Nax by protein array (View interaction) SAP97 binds to Nax by protein array (View interaction) Densin-180 binds to Nax by protein array (View interaction) Beta-1-syntrophin binds to Nax by protein array (View interaction) ERBIN binds to Nax by protein array (View interaction) Nax physically interacts with SAP97 by pull down (View interaction) Lnx1 binds to Nax by protein array (View interaction) nNOS binds to Nax by protein array (View interaction)
KW - Astrocyte
KW - MAGUK protein
KW - PDZ array
KW - PDZ-binding motif
KW - Sodium channel
KW - Subfornical organ
UR - http://www.scopus.com/inward/record.url?scp=84868103104&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868103104&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2012.09.018
DO - 10.1016/j.febslet.2012.09.018
M3 - Article
C2 - 23022437
AN - SCOPUS:84868103104
VL - 586
SP - 3805
EP - 3812
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 21
ER -