SANS investigation of assembly state of proteasome activator 28 and the 20S proteasome

Masaaki Sugiyama, Eiji Kurimoto, Hiroki Sahashi, Eri Sakata, Yukio Morimoto, Keiji Itoh, Kazuhiro Mori, Toshiharu Fukunaga, Yasufumi Minami, Koichi Kato

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The state of proteasome activator 28 (PA28) and the formation of the proteasomal complex in an aqueous solution are investigated with small-angle neutron scattering (SANS). The most appropriate state of PA28, which well reproduces the observed SANS profile, is the dissociation equilibrium between dimer and monomer with dissociation degree of 0.5. In addition, it is revealed that the packing of PA28 in the dimer is same as that in a crystal. It is also revealed that the proteasomal complex in which two PA28s connects to both basal planes of the 20S proteasome is spontaneously formed in the mixture solution of PA28 and the 20S proteasome.

Original languageEnglish
Article number012020
JournalJournal of Physics: Conference Series
Volume247
DOIs
Publication statusPublished - 2010
Externally publishedYes

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neutron scattering
assembly
dimers
dissociation
monomers
aqueous solutions
profiles
crystals

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

SANS investigation of assembly state of proteasome activator 28 and the 20S proteasome. / Sugiyama, Masaaki; Kurimoto, Eiji; Sahashi, Hiroki; Sakata, Eri; Morimoto, Yukio; Itoh, Keiji; Mori, Kazuhiro; Fukunaga, Toshiharu; Minami, Yasufumi; Kato, Koichi.

In: Journal of Physics: Conference Series, Vol. 247, 012020, 2010.

Research output: Contribution to journalArticle

Sugiyama, M, Kurimoto, E, Sahashi, H, Sakata, E, Morimoto, Y, Itoh, K, Mori, K, Fukunaga, T, Minami, Y & Kato, K 2010, 'SANS investigation of assembly state of proteasome activator 28 and the 20S proteasome', Journal of Physics: Conference Series, vol. 247, 012020. https://doi.org/10.1088/1742-6596/247/1/012020
Sugiyama, Masaaki ; Kurimoto, Eiji ; Sahashi, Hiroki ; Sakata, Eri ; Morimoto, Yukio ; Itoh, Keiji ; Mori, Kazuhiro ; Fukunaga, Toshiharu ; Minami, Yasufumi ; Kato, Koichi. / SANS investigation of assembly state of proteasome activator 28 and the 20S proteasome. In: Journal of Physics: Conference Series. 2010 ; Vol. 247.
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