Salt stress inhibits the repair of photodamaged photosystem II by suppressing the transcription and translation of psbA genes in Synechocystis

Suleyman Allakhverdiev, Yoshitaka Nishiyama, Sachio Miyairi, Hiroshi Yamamoto, Noritoshi Inagaki, Yu Kanesaki, Norio Murata

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Light stress and salt stress are major environmental factors that limit the efficiency of photosynthesis. However, we have found that the effects of light and salt stress on photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803 are completely different. Strong light induced photodamage to PSII, whereas salt stress inhibited the repair of the photodamaged PSII and did not accelerate damage to PSII directly. The combination of light and salt stress appeared to inactivate PSII very rapidly as a consequence of their synergistic effects. Radioactive labeling of cells revealed that salt stress inhibited the synthesis of proteins de novo and, in particular, the synthesis of the D1 protein. Northern- and western-blotting analyses demonstrated that salt stress inhibited the transcription and the translation of psbA genes, which encode D1 protein. DNA microarray analysis indicated that the light-induced expression of various genes was suppressed by salt stress. Thus, our results suggest that salt stress inhibits the repair of PSII via suppression of the activities of the transcriptional and translational machinery.

Original languageEnglish
Pages (from-to)1443-1453
Number of pages11
JournalPlant Physiology
Volume130
Issue number3
DOIs
Publication statusPublished - Nov 1 2002
Externally publishedYes

Fingerprint

Synechocystis
Photosystem II Protein Complex
photosystem II
translation (genetics)
salt stress
transcription (genetics)
Salts
Genes
Light
genes
D1 protein
Synechocystis sp. PCC 6803
Proteins
Photosynthesis
Cyanobacteria
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Northern blotting
Northern Blotting
Western blotting

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Salt stress inhibits the repair of photodamaged photosystem II by suppressing the transcription and translation of psbA genes in Synechocystis. / Allakhverdiev, Suleyman; Nishiyama, Yoshitaka; Miyairi, Sachio; Yamamoto, Hiroshi; Inagaki, Noritoshi; Kanesaki, Yu; Murata, Norio.

In: Plant Physiology, Vol. 130, No. 3, 01.11.2002, p. 1443-1453.

Research output: Contribution to journalArticle

Allakhverdiev, Suleyman ; Nishiyama, Yoshitaka ; Miyairi, Sachio ; Yamamoto, Hiroshi ; Inagaki, Noritoshi ; Kanesaki, Yu ; Murata, Norio. / Salt stress inhibits the repair of photodamaged photosystem II by suppressing the transcription and translation of psbA genes in Synechocystis. In: Plant Physiology. 2002 ; Vol. 130, No. 3. pp. 1443-1453.
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