Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis

Maki Katsuhara, Takeshi Otsuka, Bunichi Ezaki

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Reactive oxygen species (ROS)-related membrane lipid peroxidation in the root of Arabidopsis thaliana was fluorescently visualized and investigated under salt stress. In the control roots without salt stress, more fluorescence was observed in the elongating region than in the meristematic region. Salt stress of 100 mM NaCl enhanced the fluorescence in both, indicating that salt stress-induced ROS, and consequently membrane lipid peroxidation. In transgenic tobacco glutathione S-transferase over-expressing Arabidopsis (the parB plants), less fluorescence was observed than in the non-transgenic control plants. In the salt-stressed parB plant roots, the fluorescent brightness was reduced to 46% of that of the non-transgenic plant in the meristematic region. However, the inhibition of root growth was not improved in parB plants under salt stress at pH 5.7. That is, 100 mM of salt stress reduced the root growth to 40% or less both in the parent control plants and the parB plants. The root tissue osmotic pressure was almost the same between the two tested lines, which may be one of the reasons why no difference was observed in root growth between the two lines. These results suggested that salt stress-induced oxidative stress, and the introduction/over-expression of a glutathione S-transferase gene may have reduced the amount of ROS but the removal of ROS was not sufficient to effect salt tolerance because salt stress also caused an osmotic imbalance reducing the root growth.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalPlant Science
Volume169
Issue number2
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Lipid Peroxides
Glutathione Transferase
peroxides
glutathione transferase
Arabidopsis
Lipid Peroxidation
salt stress
Glutathione
root growth
lipid peroxidation
Salts
Lipids
Recovery
lipids
Growth
reactive oxygen species
Reactive Oxygen Species
fluorescence
Fluorescence
Membrane Lipids

Keywords

  • Arabidopsis thaliana
  • Glutathione S-transferase
  • Peroxidation
  • Reactive oxygen species (ROS)
  • Root growth
  • Salt stress

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

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title = "Salt stress-induced lipid peroxidation is reduced by glutathione S-transferase, but this reduction of lipid peroxides is not enough for a recovery of root growth in Arabidopsis",
abstract = "Reactive oxygen species (ROS)-related membrane lipid peroxidation in the root of Arabidopsis thaliana was fluorescently visualized and investigated under salt stress. In the control roots without salt stress, more fluorescence was observed in the elongating region than in the meristematic region. Salt stress of 100 mM NaCl enhanced the fluorescence in both, indicating that salt stress-induced ROS, and consequently membrane lipid peroxidation. In transgenic tobacco glutathione S-transferase over-expressing Arabidopsis (the parB plants), less fluorescence was observed than in the non-transgenic control plants. In the salt-stressed parB plant roots, the fluorescent brightness was reduced to 46{\%} of that of the non-transgenic plant in the meristematic region. However, the inhibition of root growth was not improved in parB plants under salt stress at pH 5.7. That is, 100 mM of salt stress reduced the root growth to 40{\%} or less both in the parent control plants and the parB plants. The root tissue osmotic pressure was almost the same between the two tested lines, which may be one of the reasons why no difference was observed in root growth between the two lines. These results suggested that salt stress-induced oxidative stress, and the introduction/over-expression of a glutathione S-transferase gene may have reduced the amount of ROS but the removal of ROS was not sufficient to effect salt tolerance because salt stress also caused an osmotic imbalance reducing the root growth.",
keywords = "Arabidopsis thaliana, Glutathione S-transferase, Peroxidation, Reactive oxygen species (ROS), Root growth, Salt stress",
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N2 - Reactive oxygen species (ROS)-related membrane lipid peroxidation in the root of Arabidopsis thaliana was fluorescently visualized and investigated under salt stress. In the control roots without salt stress, more fluorescence was observed in the elongating region than in the meristematic region. Salt stress of 100 mM NaCl enhanced the fluorescence in both, indicating that salt stress-induced ROS, and consequently membrane lipid peroxidation. In transgenic tobacco glutathione S-transferase over-expressing Arabidopsis (the parB plants), less fluorescence was observed than in the non-transgenic control plants. In the salt-stressed parB plant roots, the fluorescent brightness was reduced to 46% of that of the non-transgenic plant in the meristematic region. However, the inhibition of root growth was not improved in parB plants under salt stress at pH 5.7. That is, 100 mM of salt stress reduced the root growth to 40% or less both in the parent control plants and the parB plants. The root tissue osmotic pressure was almost the same between the two tested lines, which may be one of the reasons why no difference was observed in root growth between the two lines. These results suggested that salt stress-induced oxidative stress, and the introduction/over-expression of a glutathione S-transferase gene may have reduced the amount of ROS but the removal of ROS was not sufficient to effect salt tolerance because salt stress also caused an osmotic imbalance reducing the root growth.

AB - Reactive oxygen species (ROS)-related membrane lipid peroxidation in the root of Arabidopsis thaliana was fluorescently visualized and investigated under salt stress. In the control roots without salt stress, more fluorescence was observed in the elongating region than in the meristematic region. Salt stress of 100 mM NaCl enhanced the fluorescence in both, indicating that salt stress-induced ROS, and consequently membrane lipid peroxidation. In transgenic tobacco glutathione S-transferase over-expressing Arabidopsis (the parB plants), less fluorescence was observed than in the non-transgenic control plants. In the salt-stressed parB plant roots, the fluorescent brightness was reduced to 46% of that of the non-transgenic plant in the meristematic region. However, the inhibition of root growth was not improved in parB plants under salt stress at pH 5.7. That is, 100 mM of salt stress reduced the root growth to 40% or less both in the parent control plants and the parB plants. The root tissue osmotic pressure was almost the same between the two tested lines, which may be one of the reasons why no difference was observed in root growth between the two lines. These results suggested that salt stress-induced oxidative stress, and the introduction/over-expression of a glutathione S-transferase gene may have reduced the amount of ROS but the removal of ROS was not sufficient to effect salt tolerance because salt stress also caused an osmotic imbalance reducing the root growth.

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