Salt stress-induced cytoplasmic acidification and vacuolar alkalization in nitellopsis obtusa cells: In vivo 31p-nuclear magnetic resonance study

Maki Katsuhara, Kazuyuki Kuchitsu, Kazuhiko Takeshige, Masashi Tazawa

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Time courses of cytoplasmic and vacuolar pH changes under salt stress were monitored by in vivo31P-nuclear magnetic resonance spectroscopy in intact cells of Nitellopsis obtusa. When cells were treated with 100 millimolar NaCl for 2 hours, the cytoplasmic pH deceased from 7.2 to 7.0, while the vacuolar pH increased from 4.9 to 5.2. This salt-induced breakdown of the pH gradient between the cytoplasm and the vacuole was also confirmed through direct measurements of change in vacuolar pH with a micro-pH electrode. We speculate that the intracellular pH changes induced by the salt stress mainly results from the inhibition of the H+-translocating pyrophosphatase in the vacuolar membrane, since this H+-translocating system is sensitive to salt-induced increase in the cytoplasmic [Na+] and a simultaneous decrease in the cytoplasmic [K+]. Since disturbance of the cytoplasmic pH value should have serious consequences on the homeostasis of living cells, we propose that the salt-induced intracellular pH changes are one of initial and important steps that lead to cell death.

Original languageEnglish
Pages (from-to)1102-1107
Number of pages6
JournalPlant Physiology
Volume90
Issue number3
DOIs
Publication statusPublished - Jul 1 1989
Externally publishedYes

Fingerprint

alkalinization
acidification
salt stress
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Salts
cells
salts
Inorganic Pyrophosphatase
Proton-Motive Force
pyrophosphatases
Vacuoles
electrodes
Electrodes
Cytoplasm
Homeostasis
Cell Death
vacuoles
cell death
homeostasis

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Salt stress-induced cytoplasmic acidification and vacuolar alkalization in nitellopsis obtusa cells : In vivo 31p-nuclear magnetic resonance study. / Katsuhara, Maki; Kuchitsu, Kazuyuki; Takeshige, Kazuhiko; Tazawa, Masashi.

In: Plant Physiology, Vol. 90, No. 3, 01.07.1989, p. 1102-1107.

Research output: Contribution to journalArticle

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